FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804121
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGTCTGTGTGCACAGAAATATGTGGCTGTGTCCACAGGGTCCATG30560.38004230706572767No Hit
GACTTATACCACGTAGCCTCTTTGACTACTGGGGCCCGGGAACCCTGGTC23690.29460740361214294No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20730.2577970230848342No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19600.2437444115997468No Hit
CTAATGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAGGGCCCTG16380.20370068683693127No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13660.1698749317577827No Hit
CCACTAATGGAGTGGGTGTGGGCTGGATCCGTCAGCCCCCAGGAAGGGCC13320.16564671237288914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13280.1651492747981958No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12890.16029925844493553No Hit
GGATATTAACAGGTGGTTAGCCTGGTATCAACAGAAGCCAGGGACAGCCC12410.1543300075486152No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12210.1518428196751484No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10890.1354273797102675No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10500.1305773633570072No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10480.13032864456966053No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10380.12908505063292713No Hit
GTTAATATCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAG10340.12858761305823377No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9670.12025553368211997No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8930.1110529385502928No Hit
GAAATATGTGGCTGTGTCCACAGGGTCCATGTTGGTCATCGTAAGAACCA8910.11080421976294612No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8910.11080421976294612No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8900.11067986036927277No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC8810.10956062582621272No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8550.10632728159070588No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8540.10620292219703253No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG8340.10371573432356573No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8140.10122854645009892No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8120.10097982766275225No Hit
GGGTCCATGTTGGTCATCGTAAGAACCACTTGATTTTTGGAGGCGTCCTT8070.10035803069438555No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGATATTAACAG8050.10010931190703887No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC10800.068.099944
ATCAACG11350.065.4393163
CAACGCA12300.059.7876055
AACGCAG12950.056.7265436
CGCAGAG15050.048.3296628
ATAAGTC14300.042.35853
GCAGAGT17650.041.212849
TATAAGT14700.041.205892
TAAGTCT15150.039.979464
GTATAAG16850.038.5255051
ACTCGGG6500.036.561968
GACTCGG6700.035.4705547
GGACTCG6900.034.4445726
GTACATG20150.033.647941
TACATGG20150.033.6395452
TCTACAC8650.030.8448753
ACGCAGA24300.030.228937
CATGGGG12900.029.6248684
ATTACAC4400.029.4996723
GTATCAA26800.029.0666311
Produced by FastQC (version 0.11.5)