FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931623
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30520.15800184611593462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44750.079.3220141
ATCAACG54150.064.3543
TCAACGC54200.064.028944
GGTATCA22550.060.346921
TATCAAC59350.059.9286962
CAACGCA67200.051.963825
ACGCAGA66750.051.666957
CGCAGAG68700.050.3039258
AACGCAG71050.049.2493826
GCAGAGT81650.041.705049
GTACATG61650.025.1099151
GCCCATA13300.024.9026781
CCCATAA14500.024.8273642
CAGAGTA77000.024.6829389
TACATGG61400.024.27342
ATGCGGG5900.023.1856338
ACATGGG65350.022.3655223
TCCGCTA11200.021.8563828
TATACCG2003.253341E-421.5998065
CCGCTAC13050.020.4114959
Produced by FastQC (version 0.11.5)