FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730589
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC43770.25291967070170907No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT40830.23593123497260182No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC37060.2141467442587466No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT35570.205536958804199No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA35250.2036878773642962No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA34320.19831398442957862No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGG33600.1941535511897972No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGGC33590.19409576739480028No Hit
CATCTATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTG33070.19109101005495818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32820.18964641518003408No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29410.16994214108606953No Hit
CTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCAGTT26770.15468721920687117No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT26490.15306927294695621No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC25860.1494288938621475No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC24120.13937451353267588No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23870.13792991865775178No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23400.1352140802928945No Hit
GCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACCACGGT23280.13452067475293095No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23200.13405840439295524No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22820.13186262018307063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG22600.13059137669313742No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21910.12660429483834695No Hit
ATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC21780.1258531055033864No Hit
GTAAAAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG21650.1251019161684259No Hit
GTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACC21270.12290613195854129No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT21200.12250164539356254No Hit
GTCTGAGGATGAGGCTGATTTTTACTGTGCAGCATGGGATGACAGCCTCA20230.11689661727885708No Hit
GATTTTTACTGTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGG20080.11602986035390264No Hit
ACTGTAAACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAACTCCTCAT19730.11400742752900891No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC19130.1105403998291911No Hit
ATCCCATGCTGCACAGTAAAAATCAGCCTCATCCTCAGACTGGAGCCCAC18540.10713115592437025No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18380.10620661520441885No Hit
GTGTAGCACGTATCGCCAGTACAATGTTGGCCCTCCACGCCTTTCGCACA18360.10609104761442492No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA18060.10435753376451601No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC17990.10395304719953725No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG17520.10123720883467999No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36000.077.832931
GGTATCA15300.075.7967761
TCAACGC44700.061.6924254
ATCAACG45000.061.2811473
TATCAAC47050.059.223212
CAACGCA46950.058.8875735
AACGCAG50650.054.7279666
CGCAGAG56200.049.323348
ACGCAGA58450.047.547847
GCAGAGT64500.042.9763039
GTACATG51700.034.134371
TACATGG51900.033.572882
ACATGGG53550.032.00063
CAGAGTA63550.028.6637489
CATGGGG29200.024.9045734
ACGTATC10050.024.3579588
TATTGGG16100.024.149522
TAGCACG10500.024.0004484
GTATACG1550.001878698223.2356381
GTATATA8400.023.1526551
Produced by FastQC (version 0.11.5)