FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1208037
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGATAATCTCCCTCGGACTTTCGGCGGGGGGACCAAGGTGGAGATCA40090.3318606963197319No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT37260.3084342615333802No Hit
TTATCATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCT35910.29725910712999687No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34320.2840972586104565No Hit
ATATTACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGGGGGA29450.24378392383676992No Hit
GTAATACACAGCCGTGTCCTCGACTCTCAGGCGGTTCATTTGGAGATACA24910.20620229347279925No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21690.17954748074769233No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19950.1651439484055538No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19550.16183279154529207No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19010.15736272978393875No Hit
GACCAGGGAAGGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGCA18870.15620382488284712No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.15545881458928823No Hit
ACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGGGGGACCAAG18640.15429990968819665No Hit
ACATATTACTGTCAACAGTATGATAATCTCCCTCGGACTTTCGGCGGGGG17860.14784315381068625No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17700.14651869106658155No Hit
CAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT17700.14651869106658155No Hit
GTGTATTACTGTGTGAGTTCTGGTGGGGGCTACTCTGACTACTGGGGCCA17020.1408897244041366No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG16820.13923414597400577No Hit
TCCCTGGTCTCTGCTGATACCAATTTAAGTAGTTGGTAATGTCTTGACTC16340.1352607577416917No Hit
GTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA15620.12930067539322057No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG15590.12905233862870094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15560.1288040018641813No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14660.12135389892859241No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT14650.12127112000708587No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14590.12077444647804662No Hit
GTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTG14380.11903608912640921No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG14290.11829107883285031No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13740.11373823814999043No Hit
ATCATACTGTTGACAGTAATATGTTGCAAAATCTTCAGGCTGCAGGCTGC13400.11092375481876796No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCTCTT13230.10951651315315672No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC13160.10893706070261094No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAAG12800.10595701952837537No Hit
GTGGGAGACAGAGTCACCATCTCTTGCCAGGCGAGTCAAGACATTACCAA12780.1057914616853623No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12420.10281142051112672No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA12260.10148695776702203No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12160.10065916855195661No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12160.10065916855195661No Hit
CTCCTGTACAGCCTCTGGATTCAGCTTCAGTGACTACTACATGAGTTGGA12140.10049361070894351No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)