FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433978
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGCAGATACA50160.34979616144738623No Hit
GTCTATTACTGTGCGAGGTTCGCGGTGGTCACCGTCTTTGACTACTGGGG49060.3421251929945927No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28620.19958465192631966No Hit
GTACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC27430.1912860587819339No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21020.14658523352520053No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20190.14079713914718356No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19980.13933268153346845No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19760.13779848784290974No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18530.12922095039114967No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17610.12280523132154049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.12182892624573041No Hit
GTATGATAACTGGTGGGCGTTCGGCCAGGGGACCAGGGTGGAATTGAAAC17030.11876053886461298No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC16200.11297244448659605No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCT16070.11206587548762953No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15920.11101983433497585No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15860.11060141787391439No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15710.10955537672126073No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT15630.1089974881065121No Hit
TCTATGGTGCGTCCACCAGGGCCACTGATATCCCAGCCAGGTTCAGTGGC15480.10795144695385842No Hit
GATCAGTACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG14520.10125678357687495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27050.077.7784041
TCAACGC31500.065.605784
ATCAACG31950.064.6817553
GGTATCA11600.063.3556941
CAACGCA33400.062.087145
TATCAAC34700.059.763192
AACGCAG35150.059.196756
CGCAGAG42300.049.020478
GCAGAGT47100.044.0247549
GTAATAG21700.034.8636631
GTACATG41450.034.2440531
AATAGAC21400.033.6478273
CAGCCGT22000.033.0549
TACATGG42950.033.027362
ACATGGG42850.032.4322623
ACGCAGA64900.032.1720437
TAATAGA22400.032.145692
ATAGACA24000.032.102834
CATGGGA21700.030.5280364
TAGACAG24550.030.5026425
Produced by FastQC (version 0.11.5)