FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195581
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG63610.5320425801346793No Hit
CAATAATAGAAAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA42720.357315815490544No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG38470.32176824489515976No Hit
CTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA37570.3142405240631961No Hit
CTATTATTGTGCGCGACATGCACCGACGCGGGACCTTTTTAGCAGCAGCC36610.30621095517576813No Hit
ATCTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAGTCGAGT32470.2715834393487351No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC31140.26045914078594423No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCACTCAGG27620.2310173881987084No Hit
GTCTCTGGTGACTCCTTCAGCAGTGTTGATTCCTACTGGGGCTGGATCCG26330.2202276550062271No Hit
ATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC24750.20701232287900193No Hit
CTATAATAGATACTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCG23000.19237508792796137No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCA20730.17338850316289736No Hit
GTATACATAGTCTCTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGG20030.16753360918248117No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19770.1653589342754694No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19240.16092594311886857No Hit
ATAATAGATACTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA19000.15891855089701157No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18890.15799849612866046No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18420.15406735302752386No Hit
CCTCACTCACTGTGCAGAGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17660.1477106109916434No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16810.14060109687256656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16780.14035017284483442No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16650.13926283539132858No Hit
GTGCAGAGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT16510.1380918565952453No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT15990.13374250678122185No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15970.13357522409606712No Hit
ACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCACTCAGGC15390.12872402622657939No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15300.127971254143383No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15220.12730212340276403No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15150.12671663400472238No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC15130.12654935131956763No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14860.12429103506997853No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14720.12312005627389529No Hit
GTACGTAGACACGTCCAAGAACCAATTTTCCCTGAAGCTGACCTCTGTGA14710.12303641493131791No Hit
CTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCA14650.12253456687585366No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14570.12186543613523466No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14490.12119630539461568No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14420.12061081599657406No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14400.1204435333114193No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14370.12019260928368718No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14320.11977440257080031No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTACGTAGACACGTCC14200.11877070645987181No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14070.11768336900636595No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13840.11575961812708632No Hit
ATAATAGAAAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAAA13630.11400314993296147No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG13530.11316673650718773No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13320.11141026831306285No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC13310.11132662697048547No Hit
GGCTTGGGAGTATCTATTATAGTGGGAACACCTACTACAACCCGTCCCTC13190.110322930859557No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13180.11023928951697962No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13140.10990472414667012No Hit
TTCTATTATTGTGCGCGACATGCACCGACGCGGGACCTTTTTAGCAGCAG13110.109653800118938No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC13080.10940287609120586No Hit
CACCTGCACTGTCTCTGGTGACTCCTTCAGCAGTGTTGATTCCTACTGGG13060.10923559340605112No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12930.10814825595254524No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12690.10614086373068826No Hit
ATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC12630.105639015675224No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12510.10463531956429553No Hit
GGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG12400.10371526479594441No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG12260.10254428599986115No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGGCG12180.10187515525924215No Hit
GCTTAGTCCCTCCGCCGAACACCCAACCACTCAGGCTGTCATCCCATGCT12140.10154058988893265No Hit
CCTTGGGGCTGGTCGGGGATGCTGATGAGACGGTGACCGAGGTCCCTTGG12130.10145694854635529No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12040.1007041764631589No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)