FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195581
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATAGAAAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGA39070.32678672544980225No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTTGGGTGTTCGG38860.32503025725567736No Hit
CTATTATTGTGCGCGACATGCACCGACGCGGGACCTTTTTAGCAGCAGCC34510.28864627323451947No Hit
ATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC33670.28162040045802No Hit
CTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA33300.27852567078265716No Hit
ATCTATTATAGTGGGAACACCTACTACAACCCGTCCCTCAAGAGTCGAGT30430.25452060546295063No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30410.2543533227777959No Hit
GTCTCTGGTGACTCCTTCAGCAGTGTTGATTCCTACTGGGGCTGGATCCG24290.20316482112044268No Hit
CCTCACTCACTGTGCAGAGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC23420.1958880243162111No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22320.1866874766326999No Hit
GTATACATAGTCTCTTCCGATGTTGGAGCTGCTTCCAGAACAAGAGATGG21460.17949432117104572No Hit
GTGCAGAGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT21250.17773785297692082No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC20820.17414127524609374No Hit
CTATAATAGATACTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCG20390.17054469751526663No Hit
CTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCA19640.16427159682196354No Hit
GACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCAACCACTCAGG18920.15824942015639257No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18540.15507104913845235No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17360.14520137071432215No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT17220.1440303919182389No Hit
ATAATAGATACTCCCAAGCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA17010.14227392372411404No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16890.14127022761318556No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16740.14001560747452493No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15330.12822217817111514No Hit
GGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTGGCTCCAAG15300.127971254143383No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15290.12788761280080563No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15180.12696755803245452No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14920.12479288312544279No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA14900.12462560044028803No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14890.12454195909771064No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14770.12353826298678217No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14290.11952347854306818No Hit
GTACGTAGACACGTCCAAGAACCAATTTTCCCTGAAGCTGACCTCTGTGA14130.1181852170618302No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTAGTCCCTCCGCCGAACACCCA14110.11801793437667543No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTACGTAGACACGTCC14100.11793429303409805No Hit
TTCTATTATTGTGCGCGACATGCACCGACGCGGGACCTTTTTAGCAGCAG14080.11776701034894332No Hit
CATCTATAGGGATAATCAGCGGCCCTCAGGGGTCCCTGACCGGTTCTCTG13910.11634510752512794No Hit
GATTATCCCTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG13890.1161778248399732No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13870.11601054215481846No Hit
GTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTA13710.11467228067358047No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13580.11358494322007459No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13250.11082477891502124No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13180.11023928951697962No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13050.10915195206347374No Hit
ATAATAGAAAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAGGGAAA13020.10890102803574161No Hit
CACCTGCACTGTCTCTGGTGACTCCTTCAGCAGTGTTGATTCCTACTGGG12960.10839917998027737No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12840.10739548386934888No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12590.10530445030491453No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12510.10463531956429553No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12440.10404983016625388No Hit
GGCTTGGGAGTATCTATTATAGTGGGAACACCTACTACAACCCGTCCCTC12410.10379890613852177No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12300.10287885137017065No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG12260.10254428599986115No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12190.10195879660181953No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA12080.1010387418334684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17700.061.9490131
TCAACGC18750.057.6861954
ATCAACG19250.056.562443
TATCAAC20450.053.9485782
CAACGCA21350.050.327665
AACGCAG21900.049.0061726
GGTATCA7800.048.0921
ACGCAGA22750.047.4917837
CGCAGAG23650.045.684488
GTGCTAG4300.035.2301831
GAATACG3450.033.4034469
GTATACG900.00371854532.0613331
TATACGG1255.3207035E-428.8430982
ACATGGG42050.026.9225123
TATTGAG14850.026.2231985
CATGGGG21050.025.6915954
GTCCTAT3151.0968506E-925.1910461
CAGAGTA27700.024.702129
TCTACAC8200.024.6221543
TATTACT30900.024.5026284
Produced by FastQC (version 0.11.5)