FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1359642
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC43360.3189074771152995No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA38700.2846337491780925No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27110.1993907219694596No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT24430.17967965096694571No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24230.1782086755189969No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT23340.17166283477562477No Hit
CTATATTACTGTGTGAAAGACCACTTCGAGAGTGGGAGCTACGTTTTTTC20910.15379048308304685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20540.1510691785043416No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20220.14871561778762352No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20010.14717109356727728No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA19720.14503817916775152No Hit
GTAATATAGAGCCGTGTCCCCAGCTCTCAGGCTGTTCATTTGGAGATCCA18890.138933631058764No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG18660.13724200929362287No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17760.13062261977785328No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17300.12723937624757106No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16610.12216451095214768No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT16200.11914901128385266No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC16010.11775158460830129No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG15720.11561867020877553No Hit
GCTCTATATTACTGTGTGAAAGACCACTTCGAGAGTGGGAGCTACGTTTT15710.11554512143637811No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15450.11363285335404466No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15430.1134857558092498No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA15020.11047025614095475No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC14580.10723411015546741No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14230.10465990312155699No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13850.10186504977045428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27250.079.408411
ATCAACG34450.062.5912673
TCAACGC34850.061.872864
GGTATCA11900.058.1883471
CAACGCA37250.057.8864145
AACGCAG38800.055.75576
TATCAAC39450.055.3894922
CGCAGAG42550.050.597848
GCAGAGT46950.045.3892029
ATAACCG800.002090657136.058015
ACGCAGA59950.035.9545487
GTACATG42850.035.517581
TACATGG43250.033.6819342
ACATGGG44550.032.3753173
AGAGCCG11200.030.8591218
CAGCGCT15550.030.5569489
TATAGAG14550.029.2429245
CATGGGG25350.029.0170964
GTACACG23850.028.7254585
TACACGG24200.028.0099456
Produced by FastQC (version 0.11.5)