FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716062
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA62430.3637980445927944No Hit
GACTTATACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA57590.3355939354172518No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55960.32609544410400093No Hit
GTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT42760.24917514635252108No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41570.24224066496431948No Hit
GGGTAGTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC38710.22557460045149885No Hit
GTAATACACGGCCGTGTCCTCCGATCTCAGGCTGCCCAGATTCATGTACA28460.1658448237884179No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27330.1592599801172685No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT27320.15920170716442644No Hit
GTGTATTACTGTGCGAGAGGCTTCGAGGGCCTATTCGGGAGGGCCTCCGA25800.15034421833243786No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24280.14148672950044927No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT23540.13717453099013904No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23280.13565943421624627No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23060.13437742925372162No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22790.1328040595269868No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21320.12423793545920835No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG21030.12254801982678946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20840.1214408337227909No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19830.11555526548574586No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC19570.11404016871185307No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18910.11019415382427908No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18700.10897042181459643No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18300.10663950370091524No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18020.10500786102133838No Hit
GTGTACATGAATCTGGGCAGCCTGAGATCGGAGGACACGGCCGTGTATTA17820.10384240196449779No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17730.1033179453889195No Hit
CTCCTGATCCATGCTGCATCCAGTTTGCACAGTGGGGTCCCATCAAGGTT17650.10285176176618327No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17410.10145321089797454No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC17190.10017120593544988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32200.075.162521
TCAACGC37200.064.2592854
ATCAACG37600.063.3841823
CAACGCA39500.060.3353235
TATCAAC40650.058.4512982
AACGCAG41700.057.3231626
CGCAGAG49000.048.6362428
ACGCAGA53150.044.838687
GCAGAGT57550.041.785859
TACATGG48250.030.7404822
ACATGGG48050.029.9693533
GTGCTAG5550.029.8505821
CAGCGCT8250.029.6724269
GGTATCA34150.029.5294481
CATGGGA25300.027.0360874
GTACACG11000.026.8368745
GTACATG67250.025.5990331
TATGTAC13400.024.7168872
GAATACG3801.4551915E-1124.631339
ACTACCC46950.024.2304923
Produced by FastQC (version 0.11.5)