FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716062
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGA55270.3220746103579008No Hit
GACTTATACTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA52250.30447617859960774No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT50610.29491941433351476No Hit
GTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT39570.23058607439591342No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36340.2117639106279377No Hit
GGGTAGTATAAGTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC35840.2088502629858362No Hit
GTAATACACGGCCGTGTCCTCCGATCTCAGGCTGCCCAGATTCATGTACA25670.14958666994549147No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24590.1432931910385522No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACT24230.14119536473623914No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22700.13227960295140853No Hit
GTGTATTACTGTGCGAGAGGCTTCGAGGGCCTATTCGGGAGGGCCTCCGA22580.13158032751730417No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21800.1270350371956258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.12196529029836918No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT20740.1208581041943706No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19560.11398189575901105No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19470.11345743918343278No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG19290.11240852603227622No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18730.10914524067312252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18360.10698914141796742No Hit
CACCATCACCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC17700.10314312653039341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30800.071.996661
TCAACGC36650.059.1330264
ATCAACG37700.057.4860843
TATCAAC40150.055.0541952
CAACGCA40100.053.3273125
AACGCAG41500.051.701816
CGCAGAG45600.047.2110828
ACGCAGA50800.042.2367177
GCAGAGT53900.040.204759
TACATGG44900.031.4302122
ACATGGG45300.029.8811443
GGTATCA33150.029.5370861
CAGAGTA57350.026.8645769
GTACATG63550.026.5101761
GTACACG10800.026.0003035
ATACACG16450.025.824014
AGCGCTG8650.025.8037725
GTATGTA16300.024.7350461
CATGGGG31700.023.3945964
GTCTATA5250.023.31321
Produced by FastQC (version 0.11.5)