FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056343
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA47880.2328405329266567No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC45190.21975905770583995No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT40620.19753513883627394No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG33600.16339686521168892No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC28230.13728254478946364No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT26660.12964763174236982No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26090.12687572063610011No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG25920.12604901030616003No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23210.11287027504652677No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG22590.10985521384321584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22590.10985521384321584No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22590.10985521384321584No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22130.10761823295043678No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22080.10737508285339557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20710.10071277019446659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37300.070.845751
GGTATCA16700.068.5546951
TCAACGC43150.060.738854
ATCAACG44900.058.692253
CAACGCA45800.057.0672725
AACGCAG48150.054.2820636
TATCAAC49250.053.8006552
CGCAGAG55900.046.8851788
ACGCAGA59700.043.780257
GCAGAGT71900.036.1486939
GTACATG59150.032.9891471
TACATGG60150.031.2428652
ACATGGG60350.030.7814033
GTAATAC30300.029.2293421
CAGCCGT33150.027.1481849
GTATATA9800.026.4504431
AATACAC34400.026.3729113
CAGAGTA61850.025.492879
CTGTGCG31000.024.6183229
TATTGAG24600.023.7080545
Produced by FastQC (version 0.11.5)