FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1910376
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC49700.26015820969275155No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCCCGATGATCA36170.18933445562548942No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG35580.1862460583675674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34360.17985988098677957No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32690.17111814637537323No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC32190.1685008605635749No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27370.14327022533783926No Hit
CTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCTCCGGCTTC25610.13405737928030922No Hit
GTAGTAGTGATCATCGGGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTC25370.13280108209064603No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAGAGACG24000.1256297189663187No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23600.12353589031688003No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC23590.12348354460064406No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT23140.12112798737002559No Hit
CACTACTACTATCCCACACCTGACAGTAATAGTCGGCCTCATCTCCGGCT22500.11777786153092376No Hit
TAGTAGTAGTGATCATCGGGGGGTGTTCGGCGGAGGGACCAAGCTGACCG22370.11709736721985618No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21590.11301440135345084No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA20640.10804155831103406No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG20490.10725637256749455No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20170.10558130964794366No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19720.10322575241732519No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCTCCGGCTTCGACCCTGC19340.10123661520035847No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19140.10018970087563915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.080.64981
GTATCAA44500.071.685121
TCAACGC47950.064.4198764
ATCAACG47800.064.320763
TATCAAC50450.061.227612
CAACGCA55650.055.377055
AACGCAG58150.052.872446
CGCAGAG60200.050.9510278
ACGCAGA61000.050.1660967
GCAGAGT75300.040.348119
GTACATG49400.034.2551771
ACATGGG52900.032.53073
TACATGG58850.029.6087532
TATTGAG25650.026.948535
CATGGGA24400.026.2634284
GAATACG6350.026.0771339
CATGGGG30400.026.0537554
CAGAGTA71250.024.8574149
ATTGAGT17900.024.1351176
GTGCTAG7450.023.197451
Produced by FastQC (version 0.11.5)