FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350593
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCGGCCTGGAGTCCA47390.3508829084705755No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC35480.26269942166144794No Hit
CAATAGGCACGCGCAGTAATAAAAACCTGTGTCTTCAGTTCTCAGACTGT34770.25744247156619354No Hit
CATATAGAGACACCAGCCCTTTTGTGGTCTTCGGCGGAGGGACCAAGCTG33650.2491498178948062No Hit
CCATAATATGTATTACTTCCATCATATCGAATATAAGCCACCCACTCCAG32390.2398205825144955No Hit
ATATTATGGAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACCAGT30960.22923264077334918No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCAC30350.2247161061844686No Hit
GTAATAATCAGCCTCGTCCTCGGCCTGGAGTCCAGAGATGGTCAGGGAGG29400.21768215887391687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT29200.21620132786116913No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG27220.20154110083496654No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAGTGACATT24560.1818460483654217No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC24410.1807354251058609No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA24070.17821801238418977No Hit
TGCCTATTGAGTGTAAATGAAAGTACGGGGGGTTACTACTTTGACTACTG23990.17762567997909065No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC23700.17547847501060646No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23630.17496018415614475No Hit
TCTCTGGACTCCAGGCCGAGGACGAGGCTGATTATTACTGCAGCTCATAT22720.16822240304814254No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22070.16340970225671242No Hit
GATTATTACTGCAGCTCATATAGAGACACCAGCCCTTTTGTGGTCTTCGG21510.1592633754210188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21300.15770850285763363No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21180.15682000424998502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20110.14889755833178464No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19990.148009059724136No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19790.14652822871138826No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT18890.13986448915402344No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18620.13786536728681403No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18480.1368287855778906No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18300.13549603766641763No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17960.1329786249447465No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA17640.13060929532435012No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17630.13053525377371272No Hit
GTCTGAGAACTGAAGACACAGGTTTTTATTACTGCGCGTGCCTATTGAGT17160.12705530089375555No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16860.12483405437463395No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16460.12187239234913848No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16410.12150218459595155No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16260.12039156133639074No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16170.11972518738065428No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATGCCATAATTTC16150.1195771042793795No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16130.11942902117810474No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG15900.11772606551344483No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15740.11654140070324664No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG15650.11587502674751016No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15550.11513461124113629No Hit
GGTTATGACTATGTCTCCTGGTACCAACAGCATCCAGGCAAGACCCCCAA15400.11402398798157551No Hit
TTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATGACTATGTCTCCT15300.11328357247520164No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15230.11276528162073993No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15050.11143253370926698No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14910.11039595200034355No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14840.10987766114588185No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14610.10817470548122195No Hit
GGTTTTTATTACTGCGCGTGCCTATTGAGTGTAAATGAAAGTACGGGGGG14420.1067679160191116No Hit
CTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCGGCCTGGAGTCCAGA14310.10595345896210034No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG14150.10476879415190217No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCAGTAGAAGTCGGCGGTGTT14130.1046207110506274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14060.10410242019616568No Hit
GTTCTCAGACTGTTCATTTGCAGATACAGCGTGCTCTTCGACTGGTCTCT13980.1035100877910666No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13850.10254754763278057No Hit
GTATTACTTCCATCATATCGAATATAAGCCACCCACTCCAGCCCCTTGCC13840.10247350608214317No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC13580.10054842576557113No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13570.10047438421493375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5450.062.228641
TCAACGC14250.060.729164
ATCAACG14300.060.519073
CAACGCA15950.054.2524345
AACGCAG17100.052.6459166
GTATCAA17650.050.6950951
TATCAAC17150.050.4638372
CGCAGAG19100.047.131518
GCAGAGT23450.039.0042279
GTACATG28850.037.017311
TACATGG29250.035.01272
ACATGGG29150.034.63673
ACGCAGA27600.032.616377
GTACACG12550.031.0276365
TACACGG13200.030.0081736
GAATACG4900.029.3957619
TTATTGA19250.029.220984
GTGCTAG5700.029.1166841
CATGGGG20650.028.9860424
GGTTTAT13300.027.6698931
Produced by FastQC (version 0.11.5)