FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397091
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42320.17654732340157298No Hit
TCCTAGGCCAGTGTGTAGCTTGCATGCAGTAATAAACTCCAACATCCTCA35240.14701152355083724No Hit
GGCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTG32470.13545585044539402No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26980.11255309039164554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24740.10320843055186474No Hit
GTGATGGAGACACCTACTTGAGTTGGTTTCAGCAGAGGCCAGGCCAATCT24540.10237408592331289No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52050.072.526881
GGTATCA25450.064.824041
TCAACGC59000.062.7283554
ATCAACG59550.062.2699133
CAACGCA62050.059.761065
AACGCAG65000.057.045256
TATCAAC65850.056.640462
ACGCAGA72500.051.044717
CGCAGAG75500.049.111818
GCAGAGT90350.041.1194429
GTACATG64850.030.8832661
ACATGGG66000.029.0195393
TACATGG68950.028.300092
CAGAGTA90750.024.2773049
CATGGGG40050.023.371884
TATACCG2603.4043787E-622.1548825
ACGTTCG22400.020.5711049
TAGGACG24400.019.4763225
ATACCGT3751.9479558E-719.1996976
GGACGTT24050.019.159787
Produced by FastQC (version 0.11.5)