FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397091
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.164866498601847No Hit
TCCTAGGCCAGTGTGTAGCTTGCATGCAGTAATAAACTCCAACATCCTCA31230.1302829137483725No Hit
GGCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAATTCAAACGAACTGTG29820.12440078411708191No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24470.10208206530331973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.069.800331
GGTATCA24300.068.152461
TCAACGC56000.059.5286184
ATCAACG56650.059.0997773
CAACGCA58650.056.9616785
TATCAAC62400.054.1154252
AACGCAG62050.054.072566
ACGCAGA69900.048.0000347
CGCAGAG70800.047.694958
GCAGAGT85700.039.4001279
GTACATG61500.031.1434351
ACATGGG61600.028.2857343
TACATGG64450.027.928652
ACGTTCG18750.025.726419
CAGAGTA86050.023.9287939
TAGGACG22100.023.457035
GGACGTT21150.022.8085297
GACGTTC22650.021.6159118
AGGACGT23050.021.5531626
CATGGGG42200.020.9857984
Produced by FastQC (version 0.11.5)