FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501891
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGATACTTACCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCA109420.4373491890733849No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT107930.43139369381000214No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC82690.33051000223431No Hit
GATCTATGGTGCATCCAGTTTGCAGAGAGGGGTCCCATCAAGGTTCAGCG65340.2611624567177387No Hit
GTATCATATTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGACT62810.2510501057000485No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG60000.2398186012100447No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC58710.23466250128402877No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57620.2303057966953796No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA56100.22423039213139181No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55330.22115272008252956No Hit
GTGTATTACTGTGCGAGGGGGTTTGGTGGCTACGACTTCTGGGGCCAGGG54300.2170358340950905No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG50210.2006881994459391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49780.19896949947060044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG47620.19033602982703882No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAA45460.18170256018347722No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44150.17646652072372457No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43760.1749076998158593No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG43620.17434812307970252No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43420.1735487277423357No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41510.1659145022704826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41170.164555530196959No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38260.15292432803827186No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37670.15056611179303975No Hit
AGTCAGGACACAGCATGGACATGAGACTCCTCGCTCAGCTCCTGGGGCTC36740.14684892347428405No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT36740.14684892347428405No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT35550.1420925212169515No Hit
GTATTACTGTGCGAGGGGGTTTGGTGGCTACGACTTCTGGGGCCAGGGAA35480.1418127328488731No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG35260.1409333979777696No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34920.139574425904246No Hit
CCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG34810.13913475846869428No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA34480.13781575616203903No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT34030.1360171166529637No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA33050.1321000794998663No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC32930.1316204422974462No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG32340.1292622260522141No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC31910.12754352607687547No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA30600.1223074866171228No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG30350.1213082424454143No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG29600.11831050993028874No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29480.11783087272786863No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA29060.11615214251939833No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC28630.11443344254405967No Hit
TACTTACCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA28560.11415365417598129No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC28440.1136740169735612No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28330.11323434953800944No Hit
GCATGGACATGAGACTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT28320.11319437977114111No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT27700.11071625422530398No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC27630.11043646585722561No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA27060.10815818914573017No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA27040.10807824961199348No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTA26870.10739876357523169No Hit
CACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTATTACTGCCAGC26600.10631957986978649No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC26510.10595985196797143No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC26400.10552018453241967No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGG26360.1053603054649463No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG26310.10516045663060461No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26180.10464084966131618No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA25590.10228263341608408No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25440.10168308691305895No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAGCCTGAAGATTTTGCAACTTA25280.10104357064316549No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA25260.10096363110942883No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC25140.10048399390700873No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT25060.10016423577206202No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)