FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501891
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATGATACTTACCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCA99740.398658454744831No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT97970.3915838060091347No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC71740.2867431075134768No Hit
GTATCATATTGCTGGCAGTAATAAGTTGCAAAATCTTCAGGCTGCAGACT60340.2411775732835683No Hit
GATCTATGGTGCATCCAGTTTGCAGAGAGGGGTCCCATCAAGGTTCAGCG59390.23738044543107595No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT57880.23134501063395646No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG57160.22846718741943595No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA50610.20228699012067275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT48520.1939333088451895No Hit
GTGTATTACTGTGCGAGGGGGTTTGGTGGCTACGACTTCTGGGGCCAGGG48220.19273421583913927No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC45040.1800238299750069No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG42680.17059096499407847No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG40740.16283683022162038No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC38980.1558021512527924No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC38770.15496278614855724No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT38570.15416339081119043No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC37610.15032629319182972No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGCATTAGCAA37370.14936701878698952No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36840.14724862114296747No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA36630.1464092560387323No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC36220.14477049559713034No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT35690.14265209795310826No Hit
AGTCAGGACACAGCATGGACATGAGACTCCTCGCTCAGCTCCTGGGGCTC33430.13361893064086325No Hit
CCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAACTGTGG33270.1329794143709698No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT33070.132180019033603No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG32110.12834292141424228No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31490.12586479586840513No Hit
GTATTACTGTGCGAGGGGGTTTGGTGGCTACGACTTCTGGGGCCAGGGAA31270.12498546099730165No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA30530.12202769824904441No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG30160.12054881687491581No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT29490.11787084249473698No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC29200.1167117192555551No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTATTA29190.11667174948868676No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA29050.11611217275253No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28200.11271474256872101No Hit
CCATAGATCAGGGACTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGC28160.11255486350124765No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28110.11235501466690595No Hit
TACTTACCCTCATACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGAA27490.10987688912106883No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA27320.10919740308430705No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT26920.1075986124095734No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26860.10735879380836336No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA26610.10635954963665484No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26440.10568006359989304No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTA25020.10000435670458865No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39050.079.0982361
GGTATCA17850.072.201361
ATCAACG53950.056.3241923
TCAACGC54200.056.0643924
CAACGCA63450.047.6641245
AACGCAG66350.045.580846
CGCAGAG67100.044.427498
TATCAAC81100.038.0011632
ACGCAGA87800.034.2811787
GTACATG71700.032.2341651
CAGCGCT15800.031.8954059
CAGAGTA72500.031.8752949
GCAGAGT100350.029.9161599
ACATGGG73700.029.8970223
ATACACG30650.028.661794
GTACACG26300.026.8314595
ACATTAG20700.026.4373057
TATGTAC28700.026.343982
GGCAGCG21450.026.184327
GTATGTA32450.026.18171
Produced by FastQC (version 0.11.5)