FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802230
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13150.16391807835658104No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAACTCCATGTAGG11840.14758859678645775No Hit
CTACTGATGGTGAGAGTGAAATCTGTTCCAGATCCACTGCCACTGAACCT10180.12689627662889696No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9430.1175473367986737No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.1118133203694701No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8350.10408486344315221No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14650.070.8349461
TCAACGC15850.064.554664
ATCAACG16150.063.355513
CAACGCA16300.063.214545
AACGCAG17250.059.3117526
TATCAAC18600.055.0102922
ACGCAGA19500.052.4484567
CGCAGAG21350.047.8858268
GCAGAGT32750.031.217179
GAATACG3151.8189894E-1229.7131929
TATAGGA2654.8639777E-927.1908422
GTGCTAG4800.027.0242751
TACACGG8900.025.9076825
GTACATG21300.025.7132361
GGTATCA19550.025.4346081
CTAGAGA5200.024.942374
ATACACG9250.024.1484094
GTAATAC10900.023.8011951
CAGAGTA27150.023.3361599
TCTACAC6600.022.9268263
Produced by FastQC (version 0.11.5)