FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences802230
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13270.16541390872941675No Hit
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAACTCCATGTAGG11910.14846116450394525No Hit
CTACTGATGGTGAGAGTGAAATCTGTTCCAGATCCACTGCCACTGAACCT9650.12028969248220585No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8860.11044214252770403No Hit
GTATTGGAAGTAGTAGTGACCACGGGTAGTAGCCTCACGGGTATCCTCCC8290.10333694825673437No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGA8220.10246438053924684No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG11850.062.64463
TCAACGC12000.061.861544
CAACGCA12550.059.7396745
GTATCAA13300.058.553641
AACGCAG13000.056.4991656
ACGCAGA14150.051.907367
TATCAAC14350.051.7341352
CGCAGAG14850.049.945458
GTACATG22400.031.8690381
GCAGAGT26350.027.8743489
TACACGG9700.027.4981375
ATACACG10250.026.7192634
GTATTGG9200.025.8647271
ACATGGG26400.025.6618923
GTAATAC11700.023.4196011
TACATGG29800.023.2191772
GTATATA5300.023.1288931
TCTACAC7600.022.7594813
AATACAC13550.022.3395653
CAGAGTA19450.022.2134939
Produced by FastQC (version 0.11.5)