FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353745
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.14007464699871908No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43350.069.123381
TCAACGC46450.063.399094
ATCAACG46700.063.0596963
CAACGCA47050.062.59065
AACGCAG49200.060.1481366
TATCAAC55350.053.854122
ACGCAGA57100.051.699227
GGTATCA22600.050.9955561
CGCAGAG63700.046.4556548
GCAGAGT77400.038.2328839
ACATGGG66150.027.6470813
GTACATG72500.027.6202451
TACATGG78650.024.3511312
GTATTAG14500.020.8642161
CAGAGTA72500.020.7560399
GTACTAG5255.4569682E-1119.2083241
GTATATA15250.018.8934361
GTATAGG10850.018.5887011
CATGGGA36600.017.9021724
TATACTG15300.017.8828875
Produced by FastQC (version 0.11.5)