FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2353745
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28550.12129606223273974No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40450.068.000361
TCAACGC48400.056.5309264
ATCAACG49150.055.66833
CAACGCA52850.051.7709925
GGTATCA22550.049.813191
AACGCAG54950.049.6614536
TATCAAC56600.048.4681432
ACGCAGA60900.044.691247
CGCAGAG66100.041.2843678
GCAGAGT78000.034.705979
CAGAGTA72150.027.6410399
GTACATG72650.027.4543061
ACATGGG67050.027.276133
TACATGG78250.024.9363462
GTATATA16250.021.712461
CCTATAC10750.019.4239433
TATACCG3051.3541883E-518.8859165
GTATAGG10100.017.8231721
AGAGTAC65250.017.78670510-14
CCGATCG3252.3411949E-517.7221989
Produced by FastQC (version 0.11.5)