FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126151
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56250.2645625828080884No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44050.20718189818126745No Hit
GTCTTTTACTGTGCGAGACAACCTCCCTGGTTTAACTGGAGGTCGAACTG43910.20652343130850068No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA42900.20177306315496876No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT41390.1946710275986983No Hit
GTAAAAGACAGCCGTGTCTGCGGCGGTCACAGAGTAGAGATTCAGGGAGA38770.18234829040834824No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC37910.1783034224756379No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT36680.17251832066490103No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36670.17247128731684627No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA35630.16757981911915007No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTC35560.16725058568276666No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35520.1670624522905476No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAGTGACGTT34920.1642404514072613No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG33980.1598193166901128No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32070.15083594721165147No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31840.14975418020639175No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT31730.14923681337778927No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT31040.14599151236201002No Hit
ACTACAACCCGTCCCTCAGGAGTCGGGTCACCCTATCCGTCGAACCGTCC30550.14368687830732624No Hit
TCTCTACTCTGTGACCGCCGCAGACACGGCTGTCTTTTACTGTGCGAGAC29040.13658484275105578No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC28690.1349386755691388No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28590.13446834208859107No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT27670.13014127406755213No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCAAAT26070.12261593837878872No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG25850.12158120472158375No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25670.12073460445659787No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA25450.1196998707993929No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG25380.11937063736300949No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA24450.11499653599391577No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT24230.1139618023367108No Hit
GATTATTATTGCTGCTCAAATACACGCAGCAGCACTTGGGTGTTCGGCGG24080.11325630211588922No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23730.11161013493397223No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT23690.11142200154175316No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA23540.11071650132093158No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23410.11010506779621956No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22900.10770636704542623No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG22790.10718900021682375No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22710.10681273343238556No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC22490.10577799977518058No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22370.10521359959852333No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG22150.10417886594131837No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22060.10375556580882543No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA22020.10356743241660635No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCA22000.10347336572049681No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21810.10257973210745615No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC21440.10083949822942961No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG21390.10060433148915576No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC21320.10027509805277236No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40100.070.9545061
GGTATCA14950.070.8277361
TCAACGC41850.065.895674
ATCAACG43100.063.9845433
CAACGCA44050.062.7680855
TATCAAC45400.061.0587962
AACGCAG45750.060.435726
CGCAGAG54350.050.870368
GAATACG5050.044.1980869
GCAGAGT63300.043.7915349
ACGCAGA67100.041.4188427
GTACATG54800.032.7300031
GTGCTAG8000.032.414531
TACATGG54250.031.853332
GTACACG21200.030.907165
ACATGGG55700.030.3784923
TACACGG21850.029.9877246
GTGTAGA9950.029.6816481
TATTGAG24100.028.681895
CGTACAC1804.961501E-628.0013583
Produced by FastQC (version 0.11.5)