FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2126151
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50340.2367658741077186No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCGCCGCCGAACACCCA47030.22119783590158928No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT46120.2169178012286051No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC39050.18366522415388184No Hit
GTCTTTTACTGTGCGAGACAACCTCCCTGGTTTAACTGGAGGTCGAACTG38250.17990255630950014No Hit
GTAAAAGACAGCCGTGTCTGCGGCGGTCACAGAGTAGAGATTCAGGGAGA34770.1635349511864397No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA34690.16315868440200154No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC34610.1627824176175634No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA33370.15695028245877174No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT33230.15629181558600494No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT32450.15262321443773277No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC31030.14594447901395527No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAACCTGCCTCCGTGTCTGGGTCTC30870.1451919454450789No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG30490.14340467821899763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.13997124381099932No Hit
CTCCTGGACAGTCGATCACCATTTCCTGCACTGGAACCAGCAGTGACGTT28620.13460944213275539No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC27370.128730273625909No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26580.12501463912958205No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26520.12473243904125343No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26040.1224748383346244No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25720.1209697711968717No Hit
ACTACAACCCGTCCCTCAGGAGTCGGGTCACCCTATCCGTCGAACCGTCC25540.12012317093188583No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG25430.11960580410328335No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG24660.11598423630306597No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT24640.11589016960695642No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA24370.1146202692094776No Hit
TCTCTACTCTGTGACCGCCGCAGACACGGCTGTCTTTTACTGTGCGAGAC24110.11339740216005355No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23910.1124567351989581No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23900.11240970185090333No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAACCT22990.10812966717791915No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTATTGCTGCTCAAAT22940.1078945004376453No Hit
GATTATTATTGCTGCTCAAATACACGCAGCAGCACTTGGGTGTTCGGCGG21780.10243863206329185No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21590.10154499845025117No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT21460.10093356492553915No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT21350.10041619809693667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.069.534511
GTATCAA39250.067.324031
ATCAACG40850.063.1022573
TCAACGC41700.061.8159984
TATCAAC43450.059.3262832
CAACGCA44950.056.8659865
AACGCAG46100.055.603616
CGCAGAG53450.047.553338
GAATACG4950.042.1779489
GCAGAGT61800.041.0059439
ACGCAGA66500.038.221437
GTGCTAG7150.035.2456631
TATTGAG22550.028.4182515
GTACATG49550.027.0279431
TATACCG1358.4168033E-426.667985
TACATGG49700.026.0777442
GTACACG21200.025.4729545
ACATGGG50950.025.1553133
TACACGG22000.024.8739536
CTAGAGA9500.024.2538284
Produced by FastQC (version 0.11.5)