FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163987
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT65130.30097223319733435No Hit
GACTTATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCA44660.20637831927825817No Hit
ATAGTACCCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAGATCAAAGGA44510.2056851543008345No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41640.192422597732796No Hit
CACTATCACCAGTCTGCAGCCTGAAGATTTCGCAACTTACTACTGTCAAC35720.1650656866238106No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34920.16136880674421797No Hit
CTATAAGTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGCTGCAGACT32420.149816057120491No Hit
GTAATACACGGCCGTGTCCTCCGATGTCAGGCTGCCCAGACTCATGTACA31520.14565706725594932No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT28330.13091575873607372No Hit
GGGTACTATAAGTCTGTTGACAGTAGTAAGTTGCGAAATCTTCAGGCTGC27570.12740372285046073No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGGTCTGGTACAGATTTCACT26890.12426137495280701No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25900.11968648610181115No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25620.11839257814395374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25150.11622066121469306No Hit
GTGTATTACTGTGCGAGAGGGTTCGAGGGCCTTTTCGGGAGGGCCTCGGA24710.11418737728091713No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23910.1104904974013245No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23440.10831858047206384No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.10776404849012496No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22370.1033740036331087No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22090.10208009567525128No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)