FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1950620
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.15789851431852436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37150.069.193451
TCAACGC42500.059.2957534
ATCAACG44150.057.2428053
CAACGCA46500.054.504735
AACGCAG47500.053.207046
TATCAAC47900.053.0620082
GGTATCA20600.049.9832421
ACGCAGA52900.047.0951357
CGCAGAG58000.042.952918
GCAGAGT70450.035.4607939
GTACATG56200.027.0334681
TACATGG60200.025.3561822
CAGAGTA63950.024.9926539
ACATGGG57150.024.6935943
ATACCGT3509.404539E-820.5725256
GTATATA12150.019.5565971
TACCGTC2608.520126E-519.3856477
CATGGGA31350.018.1440434
AGAGTAC56750.017.7101310-14
GTATTAG11850.017.6211911
Produced by FastQC (version 0.11.5)