FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985031
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33000.1662442551275018No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42100.072.711621
TCAACGC46250.064.605454
ATCAACG48200.061.9917453
CAACGCA48650.061.4183355
GGTATCA23250.060.100171
AACGCAG51700.057.9342776
TATCAAC55250.054.731692
ACGCAGA57450.052.1358077
CGCAGAG63700.047.1334728
GCAGAGT76050.039.3846449
GTACATG63700.030.077321
ACATGGG59950.027.7431373
TACATGG63850.027.6265752
CAGAGTA70300.022.4296049
GTATATA15100.022.4190831
TATACCG2603.40525E-622.1538646
CATGGGA31200.019.8461694
CCGTATA2206.188982E-419.6358832
GTATAGA12800.019.1322421
AGAGTAC64650.018.4422410-14
Produced by FastQC (version 0.11.5)