FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526167
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44550.068.364491
TCAACGC47550.061.7799534
ATCAACG48550.060.2108423
CAACGCA53400.054.6074265
AACGCAG55250.052.648626
TATCAAC57250.051.1866652
ACGCAGA61200.047.177067
GGTATCA23950.044.493251
CGCAGAG67550.043.1685688
GCAGAGT80850.035.9753389
GTACATG79250.026.892451
ACATGGG72300.026.0916123
TACATGG84450.024.3839652
CAGAGTA74100.023.8040819
CATGGGA36500.019.5290134
TAGATCG2400.001110630518.0002275
GTATACG2801.504695E-418.0002251
CCTATAC12400.018.0002253
GTATACT11650.017.9229724
TATACTG17300.017.896185
Produced by FastQC (version 0.11.5)