FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1301107
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18160.13957345552671688No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA26550.067.024831
ATCAACG27600.062.645343
TCAACGC27800.061.9355134
CAACGCA29300.058.764755
TATCAAC31300.055.4701542
AACGCAG31250.054.8672946
ACGCAGA34650.049.880147
CGCAGAG36250.047.8661588
GCAGAGT43750.039.8250969
GGTATCA19800.039.6610871
GTACATG33850.031.286861
TACATGG37600.025.8661962
ACATGGG37500.024.974613
GAATACG2954.1445674E-721.9654469
TAGTACG2704.6974747E-621.3458214
CAGAGTA44250.021.3146179
CATGGGA19700.020.8446834
CATGGGG25650.020.2223574
GTATAGA6650.019.500881
ATACGAG4304.0252417E-818.4293863
Produced by FastQC (version 0.11.5)