FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820890
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTAAGCTGCAGGGAGA14060.17127751586692494No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13290.16189745276468223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.15337012267173433No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10030.1221844583318106No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCT9130.11122074821230615No Hit
GTGTATTACTGTGCAAGGGGATACAACTTTGCTTTTGACTACTGGGGCCA8740.1064698071605209No Hit
GTATAATCATTAGACCACTTGGACCTGTAGAATGTCCTTCCCAGCCACTC8720.10622616915786526No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGTTGCTG8400.10232796111537479No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17850.064.609231
TCAACGC18500.060.3873984
ATCAACG18950.058.9533963
CAACGCA19600.056.998315
AACGCAG20250.055.517896
TATCAAC20450.054.2767372
ACGCAGA22750.049.07937
GGTATCA8200.048.346121
CGCAGAG23800.046.5858088
GCAGAGT28650.038.699569
CTTACTC3109.1858965E-1025.5750983
CAGAGTA26800.024.7151329
GTACATG21500.023.4678021
ACAGCCG8500.022.8693988
CCGTGCA1902.2987032E-422.7356599
TACATGG22550.022.6933252
TGTAGCG1600.002250354722.5235252
CAGCCGT8350.022.4179959
TACACGA2903.4758887E-722.3681895
GTAATAC9550.021.8880671
Produced by FastQC (version 0.11.5)