FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences820890
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGGAGTCACAGAGTTAAGCTGCAGGGAGA13080.15933925373679786No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12650.1541010366797013No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGCTTATAACTATGTCTCCT9150.11146438621496182No Hit
GTATAATCATTAGACCACTTGGACCTGTAGAATGTCCTTCCCAGCCACTC8810.10732254016981568No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG8690.10586071215388176No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATTGTTGCTG8410.10244978011670261No Hit
GTGTATTACTGTGCAAGGGGATACAACTTTGCTTTTGACTACTGGGGCCA8410.10244978011670261No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12750.047.5523031
ATCAACG12200.046.7122463
TCAACGC12350.046.144894
CAACGCA12950.043.452515
AACGCAG13050.043.0537456
TATCAAC15000.039.4353832
ACGCAGA14400.039.0174567
CGCAGAG16250.035.0187458
GTAATAC8500.031.4184881
GTACATG21600.029.7398451
CAGCCGT8650.029.1459879
ACAGCCG8650.029.1459878
GCAGAGT20550.027.6912219
TACATGG21950.027.6063022
TACACAG16250.026.1932075
GTGCTAG3751.2732926E-1125.0215681
ACATGGG23450.024.6099453
GGTATCA8250.024.4966411
GATACCG1500.001537694524.0474365
AATACAC11500.023.2095833
Produced by FastQC (version 0.11.5)