FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727143
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATAACCCTGTCTCCTGGTACCAACATTACCCAGGCAAAGCCCCCACA33440.4598820314573612No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20600.2833005337327046No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18460.25387028411192847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17460.24011783101810785No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16990.23365417806401217No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16770.23062863838337164No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCTT16350.22485260808396698No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT16090.22127697027957363No Hit
CTCATTATTTATGCTGTCAGTGAGCGGCCCTCAGGGGTCCCTGATCGCTT16050.2207268721558208No Hit
TCTCTGGACTCCAGGCTGAGGATGAAGGTGATTATTACTGCTGTTCATAT16040.2205893476248826No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCTTCAGTATTTTT15650.21522589091829253No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15510.21330054748515767No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15500.21316302295421946No Hit
TTATTTATGCTGTCAGTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCT15140.208212139840444No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15050.20697441906200018No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14970.20587422281449452No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14850.20422392844323603No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14510.19954809439133706No Hit
GTCAGTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTC14490.19927304532946066No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14460.19886047173664603No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14280.1963850301797583No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT13920.1914341470659829No Hit
GACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCAAGTGGAACTG13610.1871708866068985No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13580.18675831301408388No Hit
CTCCTGCACTGGAACCTTCAGTATTTTTGATGGTTATAACCCTGTCTCCT13410.1844203959881344No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13240.18208247896218488No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13090.18001961099811178No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12960.1782317920959151No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12640.1738310071058925No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG12460.1713555655490048No Hit
ACATTACCCAGGCAAAGCCCCCACACTCATTATTTATGCTGTCAGTGAGC12060.16585458431147657No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11980.1647543880639709No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11870.16324161822365066No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11820.1625539955689596No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA11720.16117875025957754No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11650.1602160785430101No Hit
GTAATAAACAGCCGTGTCCTCGGGAGTCACAGAATTTATCTGCAGAAAGA11340.15595281808392572No Hit
GTTCATATGCAGGCAGTTCCACTTGGGTGTTCGGCGGAGGGACCAAGGTG11250.15471509730548189No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT10880.14962668966076825No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG10600.14577600279449845No Hit
CATTATTTATGCTGTCAGTGAGCGGCCCTCAGGGGTCCCTGATCGCTTCT10580.14550095373262206No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC10130.1393123498404028No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGC10070.13848720265477354No Hit
TTATAACCCTGTCTCCTGGTACCAACATTACCCAGGCAAAGCCCCCACAC10060.13834967812383533No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10060.13834967812383533No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10010.1376620554691443No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9860.13559918750507122No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9690.1332612704791217No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9620.1322985987625543No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC9610.13216107423161605No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9560.13147345157692503No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCC9530.13106087798411042No Hit
GTCAGTCACCATCTCCTGCACTGGAACCTTCAGTATTTTTGATGGTTATA9500.1306483043912958No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9480.13037325532941937No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG9180.1262475194012732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.12487227409189114No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT9050.12445970049907652No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG9020.1240471269062619No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9010.12390960237532371No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA8660.11909624379248648No Hit
GCCTTGGGCTGACGTAGGACGGTCACCTTGGTCCCTCCGCCGAACACCCA8460.11634575317372237No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG8390.11538308145715491No Hit
CTACAGGACCAAGTGGAAAAATGATTACGCAGTATCTGTGAGAAGTCGAA8190.11263259083839079No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC8140.11194496818369977No Hit
CATATGAACAGCAGTAATAATCACCTTCATCCTCAGCCTGGAGTCCAGAG8110.11153239459088515No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT7970.10960705115775025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7780.10699408506992433No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGG7700.10589388882241871No Hit
GGGTTATAACCATCAAAAATACTGAAGGTTCCAGTGCAGGAGATGGTGAC7540.1036934963274074No Hit
GTATCAACGCAGAGTACATGGGGCTGGGGTCTCAGGAGGCAGCACTCTCG7370.10135557930145789No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTG7350.10108053023958148No Hit
AATAATGAGTGTGGGGGCTTTGCCTGGGTAATGTTGGTACCAGGAGACAG7290.10025538305395226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5950.075.1550141
GTATCAA20650.070.902121
ATCAACG21950.065.617663
TCAACGC21850.065.588374
TATCAAC22000.065.468532
CAACGCA22500.063.6936075
AACGCAG23250.061.9061136
ACGCAGA26100.054.8705187
CGCAGAG26650.053.7381068
GCAGAGT29400.048.9563679
GTGTAGA5350.048.532421
CGACATA450.00992378247.9772387
TGTAGAG5800.043.4575582
GTGCTAG4750.042.5155331
GAATACG4550.041.123359
CCTATAC2005.456968E-1239.608463
CTATACC1851.03682396E-1038.927234
TCTACAC8550.035.3759773
CTATACT2501.8189894E-1234.5673834
GCTAGAG6000.033.6071783
Produced by FastQC (version 0.11.5)