FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149714
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGG42280.36774363015497763No Hit
GTGTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGTGTC33800.29398615655719595No Hit
GCCTACATGGACCTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTA30330.26380473752602823No Hit
TTCCAGGACAGAGTCACCGTGACCACAGACACGTCCACGGACACAGCCTA22630.1968315598487972No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21280.18508950921707487No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC21190.1843067058416267No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20640.17952290743611019No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19240.16734596604025No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18960.16491057776107798No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18910.1644756869969401No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA18660.16230123317625078No Hit
GTATTACTGTCAGCAGTATGGTAGCTCGCCGTACAGTTTTGGCCAGGGGA18200.15830023814618244No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC17840.1551690246443898No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG17720.15412528681045895No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17640.15342946158783838No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG17590.1529945708237005No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17550.1526466582123902No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16040.13951295713542672No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16020.13933900082977158No Hit
GTATCAACGCAGAGTACATGGGACCCAAAAACCACACCCCTCCTTGGGAG15830.1376864159260477No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA15590.13559894025818595No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15520.13499009318839295No Hit
GTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGTGTCGA15320.1332505301318415No Hit
CTCCCAGGTTCAGCTGGTGCAATCTGGAGCTGAGGTGAAGAAGCCTGGGG14920.12977140401873857No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14880.1294234914074283No Hit
CTATAACACAAACTATGCACAGAAGTTCCAGGACAGAGTCACCGTGACCA14700.12785788465653197No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG14540.1264662342112908No Hit
CATGTAGGCTGTGTCCGTGGACGTGTCTGTGGTCACGGTGACTCTGTCCT14410.12533551822453237No Hit
TCCACGGACACAGCCTACATGGACCTGAGGAGCCTGACATCTGACGACAC14330.12463969300191179No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT14060.12229128287556731No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13770.11976891644356771No Hit
GTCCTGGAACTTCTGTGCATAGTTTGTGTTATAGTTGTAAGCGCTGATCC13320.11585489956632691No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13140.11428929281543061No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC13130.11420231466260304No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13130.11420231466260304No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC12900.11220181714756887No Hit
GGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG12820.1115059919249483No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA12770.11107110116081044No Hit
GCCGTGTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGT12630.10985340702122443No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12560.10924455995143138No Hit
GGATGGATCAGCGCTTACAACTATAACACAAACTATGCACAGAAGTTCCA12180.10593939014398362No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC12010.10446076154591491No Hit
ATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGGCTG11760.10228630772522558No Hit
GTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAG11740.10211235141957044No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11730.10202537326674285No Hit
CCTTTATCAACTATGGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGG11610.10098163543281198No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC11560.10054674466867414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23400.066.9052351
TCAACGC26050.059.4674454
ATCAACG26600.058.237863
CAACGCA26750.057.6419335
AACGCAG28000.055.0111546
GTATACG450.00982734248.097771
ACGCAGA32400.047.536377
CGCAGAG32600.047.244738
GCAGAGT37450.040.934089
GGTATCA29850.034.3210221
TATCAAC48100.033.404762
GTACATG27500.031.2198281
GTATAAC2202.3750545E-829.5145421
TACACGG22450.027.6013745
CAGAGTA38150.026.9773169
ATACACG23050.026.5703074
GAATACG3057.748895E-1025.9567369
GTGCTAG4001.8189894E-1225.251331
CATGGGG15000.024.0174734
GTAATAC31800.023.3682581
Produced by FastQC (version 0.11.5)