FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149714
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGGTCCATGTAGG39330.3420850750708437No Hit
GTGTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGTGTC33080.2877237295536107No Hit
GCCTACATGGACCTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTA27880.24249509008327286No Hit
TTCCAGGACAGAGTCACCGTGACCACAGACACGTCCACGGACACAGCCTA21330.1855243999812127No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20130.17508702164190398No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18250.1587351289103203No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17890.1556039154085277No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17350.15090709515583875No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17140.14908055394645972No Hit
GTATTACTGTCAGCAGTATGGTAGCTCGCCGTACAGTTTTGGCCAGGGGA17050.14829775057101158No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA16990.14777588165404615No Hit
GTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGTGTCGA16750.14568840598618438No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG15760.1370775688562547No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15760.1370775688562547No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA15760.1370775688562547No Hit
CTCCCAGGTTCAGCTGGTGCAATCTGGAGCTGAGGTGAAGAAGCCTGGGG15720.1367296562449444No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15450.13438124611859995No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG15410.13403333350728966No Hit
GTATCAACGCAGAGTACATGGGACCCAAAAACCACACCCCTCCTTGGGAG14880.1294234914074283No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG14310.12446573669625664No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14260.12403084593211877No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC14210.1235959551679809No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14150.12307408625101549No Hit
GTCCTGGAACTTCTGTGCATAGTTTGTGTTATAGTTGTAAGCGCTGATCC14120.12281315179253276No Hit
CATGTAGGCTGTGTCCGTGGACGTGTCTGTGGTCACGGTGACTCTGTCCT14000.12176941395860189No Hit
CTATAACACAAACTATGCACAGAAGTTCCAGGACAGAGTCACCGTGACCA13890.12081265427749859No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA13830.12029078536053314No Hit
TCCACGGACACAGCCTACATGGACCTGAGGAGCCTGACATCTGACGACAC12210.10620032460246635No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT12110.10533054307419061No Hit
GGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG11930.10376493632329431No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAG11910.10359098001763917No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11900.1035040018648116No Hit
GCCGTGTATTACTGTGCGAGAGATTTGGGGTACAATGACTACGAATCGGT11600.10089465727998442No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11580.10072070097432928No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11560.10054674466867414No Hit
GACCTGGAGCATCCTTTTCTTGGTGGCAGCAGCAACAGGTGCCCACTCCC11530.10028581021019141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16150.063.7416841
TCAACGC16700.060.7167364
CAACGCA16750.060.5381285
ATCAACG17000.059.6400683
AACGCAG18150.055.880696
ACGCAGA19900.050.964347
CGCAGAG21600.045.623248
GTGCTAG4400.037.6300851
GAATACG3450.037.5294959
GCAGAGT26500.036.9158069
CCGTCGT1204.2499474E-429.9714689
GTACATG29400.029.627711
ATACCGT1856.184171E-627.2173356
TACCGTC1607.207032E-526.9731487
TACACGG22600.026.0933655
TAGTACG1959.326455E-625.8148274
TGCTAGA7100.025.3191782
TATCAAC40500.025.0341012
ATACACG23600.024.9866284
CAGAGTA26950.024.8223829
Produced by FastQC (version 0.11.5)