FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026625
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGCGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGG36470.35524169000365274No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACGCGGCCGTGTATTA27510.2679654206745403No Hit
GTGTATTACTGTGCGGCATCTCGGGTACCAGCTGCTACAGTCACCTACTA27270.2656276634603677No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24570.23932789480092537No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24380.23747717033970536No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24070.23445756727139902No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA23530.22919761353951051No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22850.22257396809935467No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20830.20289784488006818No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20800.2026056252282966No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19810.1929623767198344No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18900.18409838061609643No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAACTG17750.1728966272981858No Hit
GTTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG17750.1728966272981858No Hit
GTATTACTGTGCGGCATCTCGGGTACCAGCTGCTACAGTCACCTACTACT15840.1542919761353951No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15580.1517594058200414No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15510.15107755996590772No Hit
GATTATTACTGCTGCTCATATGGGGGCAGTTACACTTGGGTGTTCGGCGG15460.15059052721295507No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT15270.14873980275173507No Hit
CCATGTAGGCTGTGCTCGTGGATCCGTCCGCGGTAATCGTGACTCTGCCC15270.14873980275173507No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15110.14718129794228663No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG14750.14367466212102764No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14620.1424083769633508No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14300.13929136734445394No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA14280.13909655424327286No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14280.13909655424327286No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14110.13744064288323388No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14020.13656398392791916No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT14010.13646657737732862No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG14010.13646657737732862No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13960.13597954462437598No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA13420.13071959089248752No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT12840.12507001095823694No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12790.1245829782052843No Hit
CATGTAGGCTGTGCTCGTGGATCCGTCCGCGGTAATCGTGACTCTGCCCT12760.12429075855351272No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG12480.12156337513697796No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12420.1209789358334348No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12350.12029708997930112No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12190.11873858516985268No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12020.11708267380981371No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11940.1163034214050895No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT11770.11464751004505053No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCTGCTCATAT11610.11308900523560209No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11550.11250456593205893No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11470.11172531352733471No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11450.11153050042615367No Hit
CAGTAATACACGGCCGCGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTA11400.11104346767320103No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11370.11075124802142945No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC11280.10987458906611469No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11280.10987458906611469No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10920.1063679532448557No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10820.10539388773895045No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10610.10334835017654936No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10600.10325094362595884No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10500.10227687812005358No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10330.1006209667600146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.068.091531
GGTATCA5200.060.9010281
ATCAACG13850.058.6737563
TCAACGC13900.058.4655464
AACGCAG14550.058.3307766
CAACGCA14400.057.4371535
TATCAAC15200.054.4088362
CGCAGAG16900.049.7940988
CAGCGCT7050.044.8892449
GCAGCGC7850.041.230788
GGCAGCG8400.037.67497
ACGCAGA22650.037.470767
TGCACCG1352.262907E-531.9650215
GGTTTAT8600.030.128631
GTACACG13850.030.118675
TATTGAG12400.029.5805345
ATTGAGT9750.029.5076166
ACACGGG14150.029.4815467
GTACATG23500.029.4021221
ACGGGGT14300.029.17239
Produced by FastQC (version 0.11.5)