FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446766
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCGCGGCGGTCACGGACTTCAGTGTCAGGGAGA41140.2843583551175518No Hit
TCTCTAGGGTGTCTAACTTGGCAAGTGGGGTCCCATCAAGGTTCAGCGGC40640.28090237121967204No Hit
ATGTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTG40040.2767551905422162No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30300.20943262421151726No Hit
CCCTAGAGATGAGGACGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG26160.18081707753707232No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25250.17452718684293106No Hit
GTCTCTGGTGGCTACGTCAGCAGTGGTAATCTCCACTGGGCCTGGATCCG22190.15337656538790653No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA21550.14895290599862038No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20500.14169533981307275No Hit
ATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA19290.13333185878020357No Hit
GTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTGGT18330.1266963696962743No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17650.12199623159515777No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16340.1129415537827126No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16260.11238859635905184No Hit
CAGTAATACATAGCCGTGTCCGCGGCGGTCACGGACTTCAGTGTCAGGGA15470.10692814180040174No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15440.10672078276652894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15370.10623694502082577No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15280.10561486791920739No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15020.1038177562923099No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC14650.10126032820787882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23200.065.869161
TCAACGC25600.058.847174
ATCAACG26400.057.0639233
CAACGCA28300.053.4874735
AACGCAG29100.052.253886
TATCAAC29300.052.1539842
ACGCAGA32950.045.9105767
CGCAGAG33400.045.2685368
GCAGAGT41000.036.8760229
TACACGG9800.035.2975276
GTACACG9950.034.772625
ACACGGG10700.032.315167
ATAGCCG19600.031.5911948
GTAATAC21050.031.1618751
TAGCCGT20550.030.1297329
GTACATG31550.029.2445471
TATGTAC12300.028.7150942
CAGAGTA39000.027.8753939
CTGTGCG22600.027.3967259
ACGGGGT13100.026.9298159
Produced by FastQC (version 0.11.5)