FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446766
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCGCGGCGGTCACGGACTTCAGTGTCAGGGAGA39990.2764095921524283No Hit
ATGTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTG38040.2629312549506969No Hit
TCTCTAGGGTGTCTAACTTGGCAAGTGGGGTCCCATCAAGGTTCAGCGGC37120.25657224457859806No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28890.19968674961949617No Hit
CCCTAGAGATGAGGACGTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAG26180.18095531689298752No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23390.16167092674281813No Hit
GTCTCTGGTGGCTACGTCAGCAGTGGTAATCTCCACTGGGCCTGGATCCG20990.14508220403299496No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT20050.1385849543049809No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19540.1350598507291435No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19390.13402305555977953No Hit
GTATTACTGTGCGAGACATGATCATTCGGACCACGGTGCCCCCAACTGGT18770.12973763552640855No Hit
ATTAATACCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAA16840.11639753768059244No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16400.11335627185045818No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16340.1129415537827126No Hit
CAGTAATACATAGCCGTGTCCGCGGCGGTCACGGACTTCAGTGTCAGGGA15310.10582222695308018No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14730.10181328563153959No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14500.10022353303851486No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16800.051.1034131
TCAACGC16750.048.6480144
ATCAACG17300.047.521523
TATCAAC20000.041.8287242
CAACGCA19750.041.630775
AACGCAG19400.041.584976
ACGCAGA21200.038.736397
CGCAGAG21850.037.2543648
ATAGCCG19400.036.389378
TAGTACG1001.4365008E-436.0554924
TACACGG10100.035.6589666
GTACACG10200.035.3546525
TAGCCGT20450.034.873229
GTAATAC21700.034.244411
GTACATG34100.032.7936331
ACACGGG11050.032.5955167
TACATGG33900.031.059882
TACATAG25500.029.9807435
ACATGGG34550.029.6395683
CATAGCC25050.029.0444937
Produced by FastQC (version 0.11.5)