FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175936
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC61210.28130422953616285No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG55790.2563954086884908No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54490.25042096826377247No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAG51440.2364040118827024No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTAAGGCTG51240.23548486720197653No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT50170.23056744316009298No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44730.20556670784434836No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC41970.19288251125033087No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.1831395776346363No Hit
GATTATTATTGTGCAACATGGGATGACAGCCTTAATGGGGTGTTCGGCGG39220.1802442718903497No Hit
GATTATTACTATGGATGAGGAGTTTGGGGGCCGCTCCTGGGACCTGCTGG37000.17004176593429218No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT35850.16475668402011823No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC33380.15340524721315332No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA31590.1451789023206565No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC30880.14191593870407954No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT30660.14090487955528105No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30430.13984786317244624No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC29340.13483852466249008No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28960.13309214976911085No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27150.12477389040854144No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC26960.12390070296185182No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26450.12155688402600076No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTAAGGCTGT26260.12068369657931115No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA25770.11843179211153268No Hit
TCCCAGGAGCGGCCCCCAAACTCCTCATCCATAGTAATAATCAGCGGCCC25170.11567435806935498No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA24550.11282500955910467No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23830.10951608870849142No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23780.10928630253830995No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT22720.10441483573046266No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22580.10377143445395452No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)