FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175936
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC54670.2512481984764258No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC49470.22735043677755226No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGGCCGATTCTCTGGCTCCAAG48500.22289258507603166No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT46530.2138390099708815No Hit
ATAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG43760.20110885614282775No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTAAGGCTG43570.20023566869613812No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC39750.18268000529427336No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC37090.17045538104061886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36760.1689387923174211No Hit
GATTATTATTGTGCAACATGGGATGACAGCCTTAATGGGGTGTTCGGCGG36440.16746816082825966No Hit
GATTATTACTATGGATGAGGAGTTTGGGGGCCGCTCCTGGGACCTGCTGG35460.16296435189270272No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33070.15198057295802817No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC31220.14347848466131358No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC29940.1375959587046678No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28810.13240279125856644No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27560.12665813700402953No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26970.12394666019588813No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC26560.12206241360040002No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT26030.11962668019647638No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCATTAAGGCTGT24350.11190586487837878No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA23820.10947013147445514No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC23290.10703439807053147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22540.10358760551780935No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22210.1020710167946116No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22060.10138165828406719No Hit
TCCCAGGAGCGGCCCCCAAACTCCTCATCCATAGTAATAATCAGCGGCCC21980.10101400041177683No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)