FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362671
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT59380.2513257241486436No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC48120.20366779801335014No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47370.20049342460291766No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC44480.188261505728051No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA43750.18517178227523No Hit
GTCTCTGGCGGCTCCATCGCCAGTACTCGTTACTATTGGGGCTGGGTCCG43390.18364808303822241No Hit
TCCATAGACACGTCCAAGAACCAATTCTCCCTCAAACTGACGTCTGTGAT42890.18153183409793408No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42040.1779342108994439No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA40910.17315148829439223No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40400.17099291437529812No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG36950.15639079668730854No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC36130.15292014842523569No Hit
GGGTATTAGTAAGTGGTTAGCCTGGTATCAGCAGAGACCAGGCAAGGCCC36050.15258154859478953No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT34980.14805277586257248No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34950.14792580092615518No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34270.14504770236736306No Hit
CTCTGAGAGTCATGGATCTCAAGTGCAAGAAAAGGAAGCACCTGTGGTTC32600.13797943090679995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.13535528222084242No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT31130.13175765902235223No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAATCAGGGTATTAGTAA30040.12714423633252367No Hit
GTCTTTCACAATGGAAACACCTACTACAATTCGTCCCTCCAGAGTCGAGC29300.12401218790089692No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29010.12278476351552967No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG28870.12219221381224894No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28510.12066851457524133No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG28500.12062618959643556No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28210.11939876521106832No Hit
GGTCTATGCTACTTCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGCG27870.11795971593167225No Hit
CTCCAGAGTCGAGCCACCATATCCATAGACACGTCCAAGAACCAATTCTC27770.11753646614361458No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT27400.11597044192780119No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA27370.11584346699138391No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG27340.1157164920549666No Hit
CTCTATTTCTGCGCGAGACAAGATGCGACAGCAGTGGCTGATACACGGGC27250.1153355672457147No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG26520.11224584379289372No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTAGTCCCTTGGCCGAACGTC26210.11093376944991495No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT26110.11051051966185729No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA25940.10979099502215924No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25790.10915612034007274No Hit
CCATAGACACGTCCAAGAACCAATTCTCCCTCAAACTGACGTCTGTGATC25380.10742079620903631No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25230.1067859215269498No Hit
GGTATCAGCAGAGACCAGGCAAGGCCCCTAGGCTCCTGGTCTATGCTACT25050.106024071908446No Hit
GTATTAGTAAGTGGTTAGCCTGGTATCAGCAGAGACCAGGCAAGGCCCCT24980.10572779705680561No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT24480.10361154811651728No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24360.10310364837084808No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC23950.10136832423981164No Hit
GAAATAGAGTGCCGTGTCTGCGGCGATCACAGACGTCAGTTTGAGGGAGA23690.10026787479086169No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG23680.10022554981205595No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)