FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795171
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATTTCAGGCCGCTCAGCTCCATGTAGA23780.29905517178066104No Hit
GTATTACTGTCAGCAGTATGAGAGCTCACCGCTCACTTTCGGCGGAGGGA20260.2547879638467701No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19690.24761969438020248No Hit
GTCTACATGGAGCTGAGCGGCCTGAAATCTGACGACACGGCCGTGTATTA18620.23416346924120726No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA15060.18939322485352206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13270.16688234354623094No Hit
ATCTACAAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTG12870.1618519790082888No Hit
CAGTAATACACTGCAAAATCCTCAGGCTCCAGTCTGCTGATGGTGAGAGT12650.1590852785124206No Hit
GTGTATTACTGTGCGACTTTACCAGCTGCCTTCTTCTACTACTACTATGG11890.14952758589033052No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC11490.14449722135238835No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11370.14298811199100572No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT10950.13770622922616643No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTCTCCT10750.1351910469571954No Hit
GTATGAGAGCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA10610.13343041936891561No Hit
ATGCTATATCTACAAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCAGT8850.11129681540197014No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8440.10614069175057944No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8250.10375126859505691No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8210.10324823214126269No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8200.10312247302781415No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9500.066.8058243
TCAACGC9500.066.046674
CAACGCA10550.060.1682935
AACGCAG10950.057.8863756
ACGCAGA12250.051.743337
TATCAAC12700.051.1086162
CGCAGAG13250.047.8381738
GTACATG20550.036.1705171
GGACCGT1406.9531416E-736.0145346
GGTATCA4300.035.243591
TGCACCG4100.033.4277535
ACCGACC3700.033.094448
ACCGTAT900.003745412732.012928
ACATGGG21500.031.8669473
GTATCAA22500.030.7905921
CCGACCG4000.030.6123549
GCACCGA4500.030.4122736
ATACACG10600.029.9365754
TACACGG10400.029.8244635
TCGCTGC4350.028.2025871
Produced by FastQC (version 0.11.5)