FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2048923
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC118860.5801096478491383No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG85360.4166091161063642No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC71200.3474996376144931No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC68850.33603019732805967No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG66740.3257321041347088No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT63330.3090892141871608No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT61610.30069456002006906No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA58810.2870288439341059No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA56720.2768283629985119No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA53670.2619424936905877No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA52060.25408470694115887No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT51110.24944812469770705No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT50230.24515318535640432No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50020.24412825664995708No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA49560.24188317472154885No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT49070.23949167440650526No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT48740.23788107215351675No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT47170.2302185099196017No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG43090.21030560933719813No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41630.2031799145209459No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA41610.203082302263189No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG41560.20283827161879678No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC40850.19937303646842755No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA40140.19590780131805832No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA39980.1951269032560033No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC39570.19312585197198723No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT39060.1906367393991868No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCT38630.18853807585741386No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG38570.18824523908414323No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG37910.18502403457816619No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36780.17950894201490247No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTC36360.177459084602008No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG36180.17658057428219606No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA35960.17550683944687037No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA35040.1710166755900539No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT34210.16696576689314335No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.16681934850650804No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT33300.16252440916520533No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC33100.16154828658763654No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA32330.15779021466399665No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA32070.15652125531315722No Hit
TATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG31530.15388572435372144No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCT30660.14963959114129716No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG30520.148956305336999No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA30300.1478825705016733No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC29140.14222105955177428No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28620.1396831408500954No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG28390.13856059988589128No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27870.1360226811842124No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG27170.1326062521627216No Hit
GGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGT26840.13099564990973306No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT26000.12689593508394412No Hit
GTGTTGGTGCTTGTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGC25750.1256757818619831No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25440.12416279186675146No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC25210.12304025090254735No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24880.12142964864955881No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24450.1193309851077859No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG24380.11898934220563681No Hit
GTTATAACCACCAACGTCATTGCTGGTTCCAGTGCAGGAGATGGTGATCG24330.11874531156124463No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA24170.11796441349918958No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT23240.11342544351349464No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23030.11240051480704741No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22860.11157081061611393No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22590.11025304513639606No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTC22450.10956975933209788No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC22380.10922811642994881No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA22140.10805676933686625No Hit
TCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTT22110.10791035095023094No Hit
CACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAGGTC21780.1062997486972424No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC21570.10527481999079516No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21390.10439630967098323No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21330.1041034728977126No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT21330.1041034728977126No Hit
TGTCAGTAGTCGGCCCTCAGGGGTTTCGAATCGCTTCTCTGGCTCCAAGT20780.10141913580939839No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20750.10127271742276307No Hit
ATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCC20690.10097988064949244No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)