FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861994
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTC67650.36332018255697923No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44070.2366817508541918No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTT40980.22008663830280872No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.20311558469039104No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30600.16433994953796843No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA29390.1578415397686566No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA26950.14473730849830882No Hit
ATCTTATAATTACCAGTGGGGTCCGTTGAGTAACAGTAGAAGTCAGCTTC25750.13829260459485906No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT25680.13791666353382448No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACG25460.13673513448485872No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25110.13485542917968588No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC24810.13324425320382344No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24750.13292201800865094No Hit
TTATAAGATATTCGGCGGAGGGACGAAACTGACCGTCCTGGGTCAGCCCA24530.13174048895968515No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23780.12771254902002904No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG22500.12083819818968268No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG20970.11262120071278425No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA20720.11127855406623223No Hit
CCCCTGTGCTGGTCATCTATCAGGACAGTAAACGACCCTCCGGGATCCCT20720.11127855406623223No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCC20110.10800249624864527No Hit
TATCAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTC20020.10751914345588653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.10617649680933451No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19770.10617649680933451No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19660.10558573228485163No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19360.10397455630898919No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG19340.10386714457726502No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19200.10311526245519588No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG19060.10236338033312675No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG18950.10177261580864386No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18930.10166520407691969No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG18770.1008059102231264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16300.081.7574161
GTATCAA39800.078.1886441
TCAACGC50300.060.8383374
ATCAACG50850.060.3219033
TATCAAC52700.058.4760322
CAACGCA52800.057.9577335
AACGCAG55550.055.087066
CGCAGAG64350.047.440628
ACGCAGA64450.047.3670167
GCAGAGT79200.038.6364179
GTACATG57950.029.5846581
ACATGGG59850.027.5503863
CAGAGTA70650.027.2102269
TATTGAG30550.025.926145
CATGGGG42600.025.5225324
TACATGG68300.024.9845982
TTATTGA34250.023.9662824
GTACACG6850.022.0742075
TCTACAC10100.021.3873143
GTGCTAG7100.021.3061031
Produced by FastQC (version 0.11.5)