FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244221
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC42720.34334736353107687No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA39380.31650325786174643No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGGTGCTA32680.262654303375365No Hit
GTATTATTGTGCCCGGGGTGTGGTCGGGGGCTGGTTCGACCCCTGGGGCC31870.25614420589268305No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG30990.2490715073929792No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA30570.24569590129084784No Hit
GTCTTATGATAGCACCAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCG28770.23122901799599913No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28640.2301841875358156No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28250.22704969615526505No Hit
GTCTCTGGTGCCTCCACCAGCAGTAGTGGTTATTACTGGGGCTGGATCCG28230.2268889530075445No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28050.22544226467805958No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT27530.2212629428373255No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26980.21684250627501062No Hit
ATCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAG26710.2146724737807833No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCTCCATCGACAGC26410.2122613265649752No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25910.20824274787196165No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25640.20607271537773433No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC25220.20269710927560297No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA24350.19570478234975944No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23960.19257029096920883No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTGATCTATGAGGAT23860.19176657523060614No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC23760.19096285949200342No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA23000.18485461987862287No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTG22890.18397053256615986No Hit
ATACCACACTGGTGCTATCATAAGACTGACAGTAGTAGTCAGCCTCGTCC22240.17874638026524226No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22150.17802303610049983No Hit
CAATAATACACGGCCGTGTCCGCGGCGGTCACAGATGTCAGCTTCAGGGA21780.17504928786766982No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21600.17360259953818494No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21270.170950337600796No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20800.16717287362936328No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA20760.16685138733392219No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA20420.164118753822673No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG20190.16227020762388675No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20000.1607431477205416No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC19940.16026091827737998No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT19930.16018054670351972No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19510.15680494060138833No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTGGTGCTAT19450.1563227111582267No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19190.1542330502378597No Hit
CTCCTGCACCGGCAGCAGTGGCAGCATTGACGACAACTATGTGCAGTGGT19170.1540723070901391No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19130.15375082079469804No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACG19050.1531078482038159No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT18840.15142004515275018No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18770.1508574441357283No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17780.1429006583235615No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC17730.14249880045426014No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17690.14217731415881904No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17420.14000728166459173No Hit
ATAATACACGGCCGTGTCCGCGGCGGTCACAGATGTCAGCTTCAGGGAAA17340.13936430907370959No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17250.13864096490896716No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17170.13799799231808496No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA16930.13606907454543848No Hit
CACCTGCACTGTCTCTGGTGCCTCCACCAGCAGTAGTGGTTATTACTGGG16850.13542610195455632No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG16750.1346223862159536No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16600.13341681260804955No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA16390.13172900955698386No Hit
TCATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGA15820.12714782984694842No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG15790.1269067151253676No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC15700.12618337096062515No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15580.1252189120743019No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15530.12481705420500057No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15510.12465631105728003No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15500.12457593948341975No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT14930.11999475977338431No Hit
CCATATTAATAGACACATCCAACAACCAGTTTTCCCTGAAGCTGACATCT14910.11983401662566379No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT14840.11927141560864186No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG14780.11878918616548026No Hit
GACTACTACTGTCAGTCTTATGATAGCACCAGTGTGGTATTCGGCGGAGG14750.11854807144389944No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14680.11798547042687754No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14630.11758361255757618No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG14620.11750324098371591No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG14490.1164584105235324No Hit
GGTTATTACTGGGGCTGGATCCGCCAGTCCCCAGGGAGGGGGCTGGAGTG14480.11637803894967212No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14440.11605655265423104No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT14350.1153332084894886No Hit
CTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGATTCTCTGGCT14320.1150920937679078No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.11396689173386401No Hit
TTAATAGACACATCCAACAACCAGTTTTCCCTGAAGCTGACATCTGTGAC14080.1131631759952613No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTGATCTATGAGG13830.11115388664875452No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13420.10785865212048341No Hit
TCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCC13270.10665307851257935No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG13240.10641196379099853No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13230.10633159221713827No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13170.10584936277397664No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13090.10520639018309448No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA13020.10464378916607259No Hit
GTGTATTATTGTGCCCGGGGTGTGGTCGGGGGCTGGTTCGACCCCTGGGG12980.10432230287063152No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12980.10432230287063152No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12920.10384007342746988No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG12890.10359895870588906No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12840.1031971008365877No Hit
ATTAATAGACACATCCAACAACCAGTTTTCCCTGAAGCTGACATCTGTGA12730.10231301352412474No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12670.1018307840809631No Hit
CCATTGTGATCTATGAGGATAACCAAAGACCCTCTGGGGTCCCTGATCGA12590.10118781149008095No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12530.10070558204691932No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12460.10014298102989742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7550.081.217931
GTATCAA21900.077.4111941
ATCAACG26900.062.6710053
TCAACGC26950.062.5547334
CAACGCA27450.061.152845
TATCAAC28050.060.101612
AACGCAG28350.059.405876
CGCAGAG32850.050.8298878
ACGCAGA38600.043.4445347
GAATACG2900.039.708969
CAGCGCT7100.037.5067949
GTGCTAG3700.037.0451931
TATTGAG17450.035.5063555
TTATTGA18500.034.2699934
ATTGAGG8600.032.6386876
GTACATG34850.032.0855061
TTTATTG21200.031.6045913
GGCAGCG8750.031.2566227
TATGTAC15000.031.2194442
TACATGG35450.030.890892
Produced by FastQC (version 0.11.5)