FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047156
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT32590.31122392461104176No Hit
ATAATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATAGA31780.3034886874544003No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27400.26166110875552445No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27170.2594646833900584No Hit
GTATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGATGA26620.25421236186394386No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT26380.2519204397434575No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26260.25077447868321434No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA25670.24514017013701875No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25320.2417977837113095No Hit
CTATAGGAGAGTAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACC23520.2246083678076619No Hit
ATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGATGATT22940.2190695560164866No Hit
CTCCTATAGTGGTGCTCGGCCAGTATTCGGCGGAGGGACCAAGCTGACCG22740.2171596209160813No Hit
GCCGTATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGA21540.2057000103136495No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21030.20082967580761607No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG20110.1920439743457517No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGA19890.18994304573530593No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18360.17533204221720544No Hit
ATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATAGAGCG18190.17370859738186098No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17290.1651138894300372No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA16870.16110302571918605No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16830.160721038699105No Hit
ATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAACT16360.15623269121315259No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16290.15556421392801073No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT15620.149165931341653No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC15610.14907043458663274No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15290.14601453842598427No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGG14990.14314963577537634No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGCAGCTATGCCATGAACTGGG14950.1427676487552953No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14940.14267215200027503No Hit
GCCTGAGAGCCGGTGACACGGCCGTATATTATTGTGCGACCATCCATAAC14690.1402847331247684No Hit
CAATAATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATA14260.13617837265889704No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA14210.13570088888379572No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14020.13388645053841072No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAGCAGCTATGCC13970.13340896676330938No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13940.1331224764982486No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13870.13245399921310674No Hit
GTATGTGCTATCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCC13850.1322630057030662No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13600.1298755868275596No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC13460.1285386322572759No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13300.12701068417695166No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13220.12624671013678956No Hit
CTCCAGAGACAACTCCAAGAATACGCTCTATCTGCAAGTGAACAGCCTGA13170.12576922636168825No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT13170.12576922636168825No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC12980.1239547880163032No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12870.1229043237110803No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12700.1212808788757358No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT12610.12042140808055342No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT12550.11984842755043185No Hit
ATCTGCAAGTGAACAGCCTGAGAGCCGGTGACACGGCCGTATATTATTGT12540.11975293079541156No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC12440.11879796324520892No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT12360.1180339892050468No Hit
CTCCAAGAATACGCTCTATCTGCAAGTGAACAGCCTGAGAGCCGGTGACA12350.11793849245002655No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA12310.11755650542994549No Hit
ATCCATAACTGGAACGACAAGACACCCGATGATTTTGATATCTGGGGCCG11880.11345014496407413No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11870.11335464820905386No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11810.11278166767893227No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11780.11249517741387148No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG11710.11182670012872963No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11380.1086753072130609No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACTGGCCGAGCACCA11340.10829332019297985No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT11290.10781583641787854No Hit
CCACTATAGGAGAGTAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGC11250.10743384939779746No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA11210.10705186237771641No Hit
TCCTCAGGCTGCGCACCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAG11180.1067653721126556No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG10780.10294550191184504No Hit
GGGCTGGAGTGGGTCTCAACTATTAGTGGTAGTGATAGCACATACTACGC10770.10285000515682476No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10580.10103556681143974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5800.073.3911
GTATCAA14350.068.376331
TCAACGC18000.053.2653434
ATCAACG18350.052.2493743
TATCAAC18700.051.27392
CAACGCA19300.049.6775175
TCGTACG450.00985663248.061252
AACGCAG20150.047.5183076
CGCAGAG23250.041.1727078
GTACATG26850.037.618591
ACATGGG25800.036.603043
GCAGAGT26100.036.401079
TACATGG26350.036.387932
CAGCGCT7050.035.732199
TATATCG850.002818987533.9255872
CAGAGTA25250.033.0656669
GTATGTA14850.033.037051
CATGGGG15950.032.993454
GCAGCGC8200.032.4764488
TATGTAC14600.032.09572
Produced by FastQC (version 0.11.5)