FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047156
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31780.3034886874544003No Hit
ATAATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATAGA28190.2692053524021254No Hit
GTATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGATGA26730.2552628261691668No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26440.25249342027357913No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25420.24275275126151213No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT24300.2320571146992425No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT24040.22957419906871565No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23480.22422638078758084No Hit
CTATAGGAGAGTAAGCAGTAATAGTCAGCCTCATCCTCAGGCTGCGCACC23100.2205975040968108No Hit
CTCCTATAGTGGTGCTCGGCCAGTATTCGGCGGAGGGACCAAGCTGACCG22410.21400822800041255No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC22110.21114332534980462No Hit
ATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGATGATT21340.20379007521324427No Hit
GCCGTATATTATTGTGCGACCATCCATAACTGGAACGACAAGACACCCGA19320.18449973069915085No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG18280.17456806817704335No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG17940.17132117850635437No Hit
ATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATAGAGCG17380.16597336022521955No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA17030.1626309737995103No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17030.1626309737995103No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16160.1543227561127473No Hit
AGCACATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGA16040.15317679505250412No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15860.15145785346213936No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15760.1505028859119367No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG15550.14849745405651116No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14950.1427676487552953No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14380.13732433371914024No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT14370.13722883696411994No Hit
ATACTACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGACAACT14250.1360828759038768No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTTAGCAGCTATGCCATGAACTGGG14020.13388645053841072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13840.13216750894804596No Hit
CAATAATATACGGCCGTGTCACCGGCTCTCAGGCTGTTCACTTGCAGATA13570.1295890965624988No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGG13560.12949359980747854No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG13210.1261512133817693No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13160.12567372960666795No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13030.12443227179140452No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT12860.12280882695606005No Hit
GTATGTGCTATCACTACCACTAATAGTTGAGACCCACTCCAGCCCCTTCC12850.12271333020103976No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC12800.12223584642593845No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC12640.12070789834561421No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12460.11898895675524945No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTTAGCAGCTATGCC12280.1172700151648847No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12140.115933060594601No Hit
GCCTGAGAGCCGGTGACACGGCCGTATATTATTGTGCGACCATCCATAAC11830.11297266118897281No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11630.11106272608856751No Hit
GGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCTGCCCAGGGTCCA11440.10924828774318247No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11350.10838881694800011No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11220.10714735913273667No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT11200.10695636562269614No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11130.10628788833755429No Hit
CTCCAAGAATACGCTCTATCTGCAAGTGAACAGCCTGAGAGCCGGTGACA11080.10581040456245296No Hit
CTCCTGGACACCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG10970.10475994025723005No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT10840.10351848244196662No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10810.10323199217690583No Hit
CTCCAGAGACAACTCCAAGAATACGCTCTATCTGCAAGTGAACAGCCTGA10770.10285000515682476No Hit
ATCCATAACTGGAACGACAAGACACCCGATGATTTTGATATCTGGGGCCG10640.10160854734156133No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT10570.10094007005641949No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10500.10027159277127763No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)