FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970436
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTATACTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG37350.3848785494355115No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36890.3801384120127448No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGACATCATTATTA26980.2780193644918367No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACAATCTCCTGCACCCGCAGCAG24320.2506090046123598No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGAC23870.24597191365530544No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22600.23288501250984095No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21740.22402301645858153No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21700.2236108305957322No Hit
TCATTATTATGATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGGCTT21470.2212407618843489No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20860.21495492747589745No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTGATCTATGAAGAT20530.2115543941073909No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20420.2104208829845554No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19800.20403200211039163No Hit
TAATAATGATGTCCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC19440.20032232934474814No Hit
TTCCAGGACAGAGTCACAATTACCGCGGACATATCTACGGCCACAGCCTA18590.191563379759201No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGCTTTCCTGCA18250.18805979992498217No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17940.18486535948790028No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17830.1837318483650648No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17710.18249529077651694No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17670.18208310491366766No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17260.1778581998194626No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16980.17497289877951766No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16930.17445766645095606No Hit
TCTCTGAACTGAAGCCTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT16920.17435461998524374No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16920.17435461998524374No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT16820.17332415532812057No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16750.17260283006813432No Hit
CTGCACAGGTTCTTGGGCCAATTTTATACTGACTCAGCCGCACTCTGTGT16580.1708510401510249No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16540.17043885428817562No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16090.16580176333112126No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15800.16281341582546402No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15740.16219513703119012No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15420.1588976501283959No Hit
ATTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTG15250.1571458602112865No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14680.15127221166568428No Hit
GTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGAGATGGTGAGGGAGG14680.15127221166568428No Hit
GTCTTATCATAATAATGATGTCCTCTTCGGCGGAGGGACCAAGCTGACCG14150.14581074898293137No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13900.14323458734012343No Hit
GACTACTACTGTCAGTCTTATCATAATAATGATGTCCTCTTCGGCGGAGG13750.14168889035443863No Hit
ATCAGAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACGGC13740.1415858438887263No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGACATCATTATTAT13660.14076147216302776No Hit
TGATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGA13640.1405553792316031No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG13640.1405553792316031No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13500.13911272871163063No Hit
GCTATAGGTGTCCAGTCGCGGGTGCAGCTGGTGCAGTCTGGGGCTGAGGT13380.1378761711230828No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13320.13725789232880892No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13180.13581524180883645No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA12860.13251775490604223No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12620.13004463972894656No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC12580.12963245386609729No Hit
CAATTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTT12480.12860198920897412No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTT12350.12726238515471397No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12270.12643801342901542No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12210.1258197346347415No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTGATCTATGAAG12090.12458317704619366No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12070.12437708411476903No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT12050.12417099118334439No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11990.12355271238907047No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11540.11891562143201613No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11460.11809124970631757No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACAATCTCCTGCA11430.11778211030918062No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATACT11400.11747297091204366No Hit
GTCCTGGAATTTCTCTGCGTTTTTTGGTATTCCAAACTCAGGAATGATCC11280.11623641332349584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11160.114999855734948No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11040.11376329814640018No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT11000.1133511122835509No Hit
GTTCAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCCGTCGATGGAGCCA10830.11159932236644148No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT10810.11139322943501684No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10710.11036276477789364No Hit
CTGTGTCGGAGTCTCCGGGGAAGACGGTAACAATCTCCTGCACCCGCAGC10700.11025971831218134No Hit
CTACTACTGTCAGTCTTATCATAATAATGATGTCCTCTTCGGCGGAGGGA10640.10964143951790742No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10560.10881706779220886No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT10420.1073744172722364No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10390.10706527787509942No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10200.10510739502656537No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10180.10490130209514074No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10090.10397388390372987No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10030.10335560510945595No Hit
CCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACGGCTCCTCCAAT10000.10304646571231901No Hit
ACTATGGGTCGGGGAATCATGCTTTTTTTGTCTGGTTCGACCCCTGGGGC9900.10201600105519582No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9900.10201600105519582No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGAGATGGTGAGGGA9820.10119162932949725No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9800.10098553639807262No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9730.1002642111380864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.085.413881
GGTATCA5950.077.599591
ATCAACG20000.063.807333
TCAACGC20250.063.3723684
TATCAAC20150.063.3323442
CAACGCA21100.060.4777645
TATTGAG12350.043.7823685
GCAGAGT30150.041.777179
AACGCAG31150.040.671266
ATTGAGT10350.037.5566146
TTATTGA14150.037.1938674
GTACATG24700.036.801881
CGCAGAG34900.036.29748
GGTTTAT10700.035.7345661
ACATGGG26450.033.527933
ACGCAGA38650.032.7756587
CAGCGCT6550.030.7684069
CATGGGG19950.030.3557724
GAGTGCA13750.029.31399
TTTATTG18400.028.9962083
Produced by FastQC (version 0.11.5)