FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970436
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36470.3758104604528274No Hit
ATTTTATACTGACTCAGCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACG35200.3627235593073629No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGACATCATTATTA27470.2830686413117403No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGAC23710.2443231702039083No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACAATCTCCTGCACCCGCAGCAG23420.2413348226982511No Hit
TCATTATTATGATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGGCTT22820.23515203475551194No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22160.2283509680184989No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20130.20743253547889812No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20100.20712339608176117No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19880.20485637383609018No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19490.20083756167330974No Hit
TAATAATGATGTCCTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTC18720.19290298381346116No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18510.19073900803350247No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17670.18208310491366766No Hit
TTCCAGGACAGAGTCACAATTACCGCGGACATATCTACGGCCACAGCCTA17470.1800221755994213No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTGATCTATGAAGAT17350.17878561801087345No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGCTTTCCTGCA16830.1734272017938329No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16610.17116017954816187No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16360.16858401790535388No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16330.16827487850821693No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16070.16559567039969664No Hit
TCTCTGAACTGAAGCCTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT15870.16353474108545024No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15800.16281341582546402No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15650.16126771883977922No Hit
CTGCACAGGTTCTTGGGCCAATTTTATACTGACTCAGCCGCACTCTGTGT15500.15972202185409445No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15420.1588976501283959No Hit
ATTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTG15100.1556001632256017No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15080.15539407029417704No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15050.1550849308970401No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14990.1544666521027662No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14800.15250876925423212No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14710.15158135106282125No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14590.1503447934742734No Hit
GTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGAGATGGTGAGGGAGG14510.14952042174857486No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14500.14941737528286256No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13790.1421010762172879No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGAGGACATCATTATTAT13680.1409675650944524No Hit
GACTACTACTGTCAGTCTTATCATAATAATGATGTCCTCTTCGGCGGAGG13650.14065842569731543No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13620.14034928630017848No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13350.13756703172594587No Hit
ATCAGAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACGGC13120.13519696301456252No Hit
GTCTTATCATAATAATGATGTCCTCTTCGGCGGAGGGACCAAGCTGACCG13090.13488782361742557No Hit
TGATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGA12990.13385735896030238No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12450.12829284981183714No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12370.12746847808613862No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG12260.1263349669633031No Hit
GTTCAGAGATGGTGAGGGAGGCAGAATTGGAGGAGCCGTCGATGGAGCCA12250.12623192049759077No Hit
CAATTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTT12110.1247892699776183No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTTTGATCTATGAAG11620.11973999315771468No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11560.11912171436344075No Hit
GCTATAGGTGTCCAGTCGCGGGTGCAGCTGGTGCAGTCTGGGGCTGAGGT11480.1182973426377422No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11370.1171638315149067No Hit
GTCCTGGAATTTCTCTGCGTTTTTTGGTATTCCAAACTCAGGAATGATCC11310.11654555272063277No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA11140.11479376280352335No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10970.11304197288641393No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10780.11108409003787988No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCTTTTATTT10660.10984753244933204No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10450.10768355666937335No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10430.10747746373794873No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10340.10655004554653784No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10290.10603481321797625No Hit
GCCGCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACAATCTCCTGCA10270.1058287202865516No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10130.10438606976657915No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10070.10376779097230523No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9940.10242818691804509No Hit
CAGTAGTAGTCAGCCTCGTCCTCAGGCTTCAGTTCAGAGATGGTGAGGGA9890.10191295458948349No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT9810.10108858286378493No Hit
CTACTACTGTCAGTCTTATCATAATAATGATGTCCTCTTCGGCGGAGGGA9780.100779443466648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)