FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3235592
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC196320.6067514074704103No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC167990.5191940145729127No Hit
ATCATAGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCC135620.419150498579549No Hit
CCCTATGATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCG135150.41769790505106946No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCTATGATTACCA111520.3446664474383668No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC97770.3021703601690201No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA86510.267369927976086No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA84290.26050874152241693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT83870.25921067922037144No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCT79990.24721905604909397No Hit
CCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGTGTCTCATCAGT78050.24122324446345522No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC73360.22672821542394717No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACG69110.21359306117705815No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG68460.21158415523341634No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTGGTGT65680.20299221904368658No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC65510.20246681287381102No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA63880.197429094892063No Hit
GTAATACATGGCCGTGTCCTCAGGTCTCAGCCTGGTCAACTCCATGTAGA63780.19712003243919507No Hit
TCTATGAGGACGCTAAACGACCCGCCGGGACCCCTGAGAGATTCTCTGGC62750.1939366891746549No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG61160.1890225961740541No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG61030.1886208149853257No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA61010.18855900249475213No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT58850.18188325351280385No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA58080.17950347262572042No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCC56630.17502206705913476No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG56340.17412578594581765No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG56110.1734149423042213No Hit
GGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA55990.17304406736077974No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACCCTATGATTACCAC55180.17054066149254912No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT53640.16578109971838229No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG52940.16361766254830648No Hit
GGGCCACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACA51820.16015616307618513No Hit
GACTACTATTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGG51260.15842541334012447No Hit
GTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG51010.15765275720795452No Hit
CCCCCATGCTGGTCATCTATGAGGACGCTAAACGACCCGCCGGGACCCCT50320.1555202262831655No Hit
GTCAGGGACAATGGCCACCTTGACCATCAGTGGGGCCCAGGTGGAGGACG49360.1525532267356329No Hit
GCTCTGGAGATGCGTTCCCAAAAAAATATGCTTATTGGTACCAGCAGAAG49330.15246050799977254No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT49280.15230597677333854No Hit
ATGTATTACTGTGCGAGAGATGTGATTGGGAGTAGCAGTTGGTTCGTGGG48730.15060613328256467No Hit
CACCAGTGTGGCCCTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA48550.15004982086740232No Hit
ATAGTAGTCAGCGTCGTCCTCCACCTGGGCCCCACTGATGGTCAAGGTGG47620.14717554005573014No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47170.14578475901782423No Hit
CTACTATTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGGCG45460.14049979107378185No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTGATGAGACACAC44740.13827454141313245No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC43690.13502938565801867No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC42670.1318769486387653No Hit
ACACTGGTGTGTCTCATCAGTGACTTCTACCCGGGAGCCGTGACAGTGGC42640.13178422990290495No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG41390.12792094924205524No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCGTTCCCAAAA38740.11973079424105389No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC38700.11960716925990668No Hit
GTCCCTGACATGGAGCCAGAGAATCTCTCAGGGGTCCCGGCGGGTCGTTT38540.11911266933531792No Hit
ATGATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCGTCCT38120.11781460703327244No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG37590.11617657603307215No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT37520.11596023231606456No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAGGGCCACACTG36380.11243692035336965No Hit
CTATTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGGCGGAG36210.11191151418349408No Hit
GATTACCACTGCTGTCTGCTGAGTAGCAATAGTAGTCAGCGTCGTCCTCC36130.11166426422119971No Hit
GTCTGGGGCTGAGGTGAAGAAGCCAGGGGCCTCAGTGAAGCTCTCCTGTA35840.11076798310788258No Hit
TCATAGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCA35470.10962445203227106No Hit
GTGGTAATCATAGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGT35450.10956263954169747No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35310.1091299521076823No Hit
GGTAATCATAGGGTGTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCA34740.10736829612633485No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34400.10631748378658373No Hit
TTGCTACTCAGCAGACAGCAGTGGTAATCATAGGGTGTTCGGCGGAGGGA34160.10557573389970058No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33800.10446310906937585No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA33740.10427767159765508No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC33160.10248510937102082No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33070.10220695316343964No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32950.10183607821999806No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA32710.10109432833311494No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62500.077.570311
GGTATCA21800.075.672741
ATCAACG73850.064.837093
TCAACGC74350.064.401064
TATCAAC78200.061.597772
CAACGCA80350.059.59115
AACGCAG82900.057.8422556
CGCAGAG91200.052.4991538
GAATACG7450.052.1870779
ACGCAGA100750.047.5942737
GCAGAGT102950.046.5072679
TATTGAG63750.038.288225
GTGCTAG10900.037.0102461
TTATTGA66750.036.4600944
GTACATG90700.034.311281
ATTGAGT47600.032.5204856
ACATGGG93750.032.1038673
GGTTTAT51650.031.2419511
CTAGAGA13300.030.8585534
GTAATAC38900.030.7410681
Produced by FastQC (version 0.11.5)