FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1862090
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC40180.21577904397746617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32300.1734610034960716No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG29780.15992782303755457No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA26010.13968175544683661No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25680.13790955324393558No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC24210.1300151979764673No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG23760.12759855860887498No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT22540.12104678076784688No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21900.11760978255616No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21830.11723386087675676No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA20450.10982283348280694No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT20240.10869506844459721No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19790.10627842907700487No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19040.10225069679768432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34550.081.893011
GGTATCA14800.076.373081
ATCAACG46250.060.247163
TCAACGC46950.059.5022624
TATCAAC49700.056.4996262
CAACGCA52450.052.713655
AACGCAG54950.050.315396
CGCAGAG59550.046.1869168
ACGCAGA62250.043.9522977
GCAGAGT70950.038.6601269
GTAATAC20800.029.7671681
AATCCGC6100.029.5085286
CAGAGTA65300.028.113689
CAGCCGT20550.028.0264999
TACATGG53600.027.6719512
GTACATG54250.027.4709051
ACATGGG54950.026.7300533
AATACAC23900.025.3057213
TATTGAG26900.024.0894875
CTGTGCG19200.023.2477639
Produced by FastQC (version 0.11.5)