FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998009
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCTACATGGGATGACAGCCTGAGTGGTCCGGTGTTCGG33700.3376723055603707No Hit
GTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCTGGGACCC32340.32404517394131716No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCACCCTCAGCGTCT30500.3056084664567153No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28630.28687116048051675No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25670.2572121093096355No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC25150.25200173545529153No Hit
CCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25090.251400538472098No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA24700.24749275808133997No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA24180.24228238422699597No Hit
CTTTTATTACTGTGCGAGAGGCCCCAATCTTCAAGTCATGCCAGCATCTC24110.24158098774660347No Hit
GTAATAAAAGCCCGTGTCGTCGGCTCTCAAATTGTGCATTTGAAGATACA23950.23997779579142073No Hit
CTACTATATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGCCTGGAATGGG21550.2159299164636792No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21420.21462732300009316No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC21110.2115211385869266No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGATTCAGCCAC21000.21041894411773843No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG20880.20921655015135135No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19900.19939699942585687No Hit
GACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCACTCAGG19870.1990964009342601No Hit
GTGATAATCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGGCTCCAAG19820.19859540344826548No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19800.19839500445386765No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19710.19749320897907732No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18570.18607046629840013No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT18090.18126089043285182No Hit
GCCTTGGGCTGACCTAGGGCGGTCAGCTTGGTCCCTCCGCCGAACACCGG17630.17665171356170134No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17510.1754493195953143No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16510.16542936987542198No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16390.1642269759090349No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16310.1634253799314435No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16010.1604193950154758No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15840.15871600356309412No Hit
GTGCTACATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCGGAGGGACC15700.15731321060230918No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15690.15721301110511027No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15440.1547080236751372No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT15430.15460782417793828No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15350.1538062282003469No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15260.15290443272555657No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA14740.1476940588712126No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14570.14599066741883088No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC14120.14148169004487937No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14000.14027929607849227No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA13770.13797470764291705No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT13620.1364717151849332No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG13420.13446772524095474No Hit
GTGCTGATTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAAAGGGTCAC13200.13226333630257842No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13130.13156193982218597No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC12810.12835555591182043No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12670.12695276295103552No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC12590.1261511669734441No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12530.1255499699902506No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12310.12334558105187429No Hit
CTCCTGTGCAGCCTCTGGGTTCAGCGTCAGTAACTACTATATGAGCTGGG12150.12174238909669151No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT12060.12084059362190121No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGAGGAGGCT11860.11883660367792274No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11840.1186362046835249No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11840.1186362046835249No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT11760.1178346087059335No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11730.11753401021433675No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC11710.11733361121993889No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11660.1168326137339443No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT11270.11292483334318629No Hit
TTATTACTGTGCGAGAGGCCCCAATCTTCAAGTCATGCCAGCATCTCCCC11220.11242383585719168No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11040.11062024490761106No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGGTTCAGCGTCAGTAACTACTAT11010.11031964641601429No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10920.10941785094122397No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG10810.10831565647203582No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10700.10721346200284766No Hit
AGAATACACTGTATCTTCAAATGCACAATTTGAGAGCCGACGACACGGGC10680.10701306300844983No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGAGGAGGCTTGATCCAGCCGGGGG10670.1069128635112509No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC10670.1069128635112509No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA10550.10571046954486382No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG10500.10520947205886921No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10490.10510927256167028No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10430.10450807557847676No Hit
TTATTACTGTGCTACATGGGATGACAGCCTGAGTGGTCCGGTGTTCGGCG10430.10450807557847676No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA10400.10420747708687998No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10210.10230368664010045No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG10120.10140189116531013No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10110.10130169166811122No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10040.10060029518771875No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10030.10050009569051983No Hit
GATTATCACTATGGATGAGGAGTTTGGGGGCCGTGCCGGGGAATTGCTGG10030.10050009569051983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)